TH1/TH2 balance in concomitant immediate and delayed-type hypersensitivity diseases
TH1/TH2 balance in concomitant immediate and delayed-type hypersensitivity diseases
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DOI：
10.1016/j.imlet.2009.04.011
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发表时间：
2009-06-04
影响因子：
4.4
通讯作者：
Rodrigues, Virmondes
中科院分区：
文献类型：
--
作者：
de Mello, Luane Marques;Silveira Bechara, Monique Isabel;Rodrigues, Virmondes
尽管观察到TH1和TH2 CD4+细胞的相互抑制特性，但一组患者同时出现了即时和延迟型超敏反应，理论上是拮抗的。同时出现过敏反应的患者评估细胞因子的产生。对45例患者和13例非特应性个体的PBMC进行螨变应原和丝裂原培养，用ELISA法检测细胞因子的产生。细胞因子水平的分析显示，在特异性和丝裂原刺激后，非特应性个体的促炎细胞因子tnf - α的产生增加。血清样品中IL-4含量较高，双致敏组（DerpNi组）经PHA刺激后IL-5水平较高。在PHA刺激后，致敏组（Derp组和DerpNi组）IL-13水平升高。特应性患者（Derp组和DerpNi组）的ifn - γ水平最低，对Derp I刺激后tgf - β产生的分析显示，对翼状螨致敏患者的ifn - γ水平升高。抗原刺激后IL-10水平无差异，但Derp和DerpNi组的基础产量较高。此外，ifn - γ水平与IL-4、IL-13和IL-10呈负相关。该研究表明，患者能够同时对两种抗原（rDER P I和NiSO4）产生反应，并呈现出不同的细胞因子产生模式。螨虫抗原（rDER p1）敏感个体IL-13和NiSO4敏感个体ifn - γ水平的升高证实了TH2型反应在异位和TH1型在DCA中的作用。(C) 2009 Elsevier B.V.版权所有
In spite of the observation of mutual inhibitory properties of TH1 and TH2 CD4+ cells, a group of patients developed simultaneously immediate and delayed-type hypersensitivity reactions that are theoretically antagonistic. Patients presenting concomitant hypersensitivity reactions were evaluated for cytokine production.PBMC from 45 patients and 13 non-atopic individuals were cultured with mite allergen and mitogen and the supernatants obtained were evaluated for cytokine production by ELISA.The analysis of the cytokines levels revealed increased production of pro-inflammatory cytokine TNF-alpha in the non-atopic individuals after specific and mitogen stimulus. The IL-4 was largely observed on serum samples and IL-5 levels were higher in the double sensitized group (group DerpNi) after PHA stimulus. The IL-13 levels were increased in sensitized groups (Derp and DerpNi groups) after PHA stimuli. Atopic patients (Derp and DerpNi groups) presented lowest levels IFN-gamma and the analysis of TGF-beta production after rDER P I stimulation have shown increased levels among sensitized patients to Dermatophagoides pteronyssinus mite. IL-10 levels did not differ after antigen stimulation but basal production was higher on Derp and DerpNi groups. Furthermore, negative correlations were observed between IFN-gamma levels and IL-4, IL-13 and IL-10.This study has shown patients able to react, concomitantly, to the two types of antigens - rDER P I and NiSO4, present distinct pattern of cytokine production. The increased levels of IL-13 in the sensitive individuals to mite antigen (rDER P 1) and IFN-gamma in NiSO4 sensitized individuals confirm the role of the type TH2 response in the atopies and TH1 type in DCA. (C) 2009 Elsevier B.V. All rights reserved.