A combined stress response analysis of Spirulina platensis in terms of global differentially expressed proteins, and mRNA levels and stability of fatty acid biosynthesis genes
A combined stress response analysis of Spirulina platensis in terms of global differentially expressed proteins, and mRNA levels and stability of fatty acid biosynthesis genes
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DOI：
10.1111/j.1574-6968.2008.01100.x
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发表时间：
2008-04-01
影响因子：
2.1
通讯作者：
Hongsthong, Apiradee
中科院分区：
文献类型：
--
作者：
Jeamton, Wattana;Mungpakdee, Sutada;Hongsthong, Apiradee
基因表达的变化在提高蓝藻在寒冷条件下的生存能力方面起着关键作用。在本研究中，螺旋藻培养物在最佳生长温度下生长，在光照下，然后转移到22摄氏度的黑暗条件下。然后进行二维差异凝胶电泳以分离随后通过MS鉴定的差异表达的蛋白质。在鉴定的所有差异蛋白质中，参与脂肪酸生物合成的蛋白质，由fabZ编码的（3R）-羟基肉豆蔻酰-[酰基-载体-蛋白]-脂肪酶是上调最多的蛋白质。然而，脂肪酸去饱和蛋白没有显着分化。这就提出了一个问题：与冷转移相比，冷暗转移中细胞中的不饱和脂肪酸，特别是γ-亚麻酸含量如何保持稳定。因此，在这些去饱和酶基因desC、德萨和desD以及fabZ基因的转录水平上进行了研究。结果表明，在黑暗中，能量是有限的，mRNA的稳定性增强暴露于低温。这些数据表明，当细胞遇到能量有限的冷应激时，它们可以通过mRNA稳定性来维持其稳态粘性适应能力。
Changes in gene expression play a critical role in enhancing the ability of cyanobacteria to survive under cold conditions. In the present study, Spirulina platensis cultures were grown at the optimal growth temperature, in the light, before being transferred to dark conditions at 22 degrees C. Two dimensional- differential gel electrophoresis was then performed to separate differentially expressed proteins that were subsequently identified by MS. Among all differentiated proteins identified, a protein involved in fatty acid biosynthesis, (3R)-hydroxymyristoyl-[acyl-carrier-protein]-dehydratase encoded by fabZ, was the most up-regulated protein. However, the fatty-acid desaturation proteins were not significantly differentiated. This raised the question of how the unsaturated fatty acid, especially gamma-linolenic acid, content in the cells in the cold-dark shift remained stable compared with that of the cold shift. Thus, a study at the transcriptional level of these desaturase genes, desC, desA and desD, and also of the fabZ gene was conducted. The results indicated that in the dark, where energy is limited, mRNA stability was enhanced by exposure to low temperatures. The data demonstrate that when the cells encounter cold stress with energy limitation, they can maintain their homeoviscous adaptation ability via mRNA stability.