Establishment of the male germline and sperm cell movement during pollen germination and tube growth in maize
Establishment of the male germline and sperm cell movement during pollen germination and tube growth in maize
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DOI：
10.4161/psb.5.7.12033
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Dresselhaus, Thomas
中科院分区：
文献类型：
--
作者：
Kliwer, Irina;Dresselhaus, Thomas
开花植物（被子植物）需要两个精子才能实现双受精。与动物和低等植物（如苔藓和蕨类植物）不同，开花植物（被子植物）的精子细胞是不动的，通过花粉管运送到雌性配子（卵子和中心细胞）上。这两个精细胞是由生殖花粉细胞在花粉粒内或在花粉管内萌发后产生的。虽然烟草和百合等模式植物的花粉管生长和精子行为已经得到了深入的研究，但对禾草植物花粉萌发、花粉管生长和精子释放过程中精子的动力学和行为知之甚少。在《实验植物学杂志》3月刊上，我们报道了孢子体和配子体对玉米花粉管萌发、生长和引导的控制。(1)精子在雌性配子体内传递前，存在不同的合子前交叉障碍，可分为5个程序期。利用活细胞成像和生殖细胞特异性启动子驱动α -微管蛋白- yfp在雄性种系中的表达，我们报道了玉米花粉粒内雄性种系的形成以及花粉萌发过程中精子的行为和它们在管状生长过程中的运动动态。
Two sperm cells are required to achieve double fertilization in flowering plants (angiosperms). In contrast to animals and lower plants such as mosses and ferns, sperm cells of flowering plants (angiosperms) are immobile and are transported to the female gametes (egg and central cell) via the pollen tube. The two sperm cells arise from the generative pollen cell either within the pollen grain or after germination inside the pollen tube. While pollen tube growth and sperm behavior has been intensively investigated in model plant species such as tobacco and lily, little is know about sperm dynamics and behavior during pollen germination, tube growth and sperm release in grasses. In the March issue of Journal of Experimental Botany, we have reported about the sporophytic and gametophytic control of pollen tube germination, growth and guidance in maize.(1) Five progamic phases were distinguished involving various prezygotic crossing barriers before sperm cell delivery inside the female gametophyte takes place. Using live cell imaging and a generative cell-specific promoter driving alpha-tubulin-YFP expression in the male germline, we report here the formation of the male germline inside the pollen grain and the sperm behaviour during pollen germination and their movement dynamics during tube growth in maize.