Microplate measurement of urinary albumin and creatinine.

Microplate measurement of urinary albumin and creatinine.
复制标题

DOI：
10.1093/clinchem/35.7.1371
复制
发表时间：
1989-07
期刊：
Clinical chemistry
影响因子：
9.3
通讯作者：
R. Magnotti;G. Stephens;R. K. Rogers;A. Pesce
R. Magnotti;G. Stephens;R. K. Rogers;A. Pesce
中科院分区：
医学1区
文献类型：
--
作者：
R. Magnotti;G. Stephens;R. K. Rogers;A. Pesce

文献摘要

被引文献

相似文献

我们描述了用改良的商业酶试剂盒检测人尿白蛋白(HUA)和肌酐的竞争酶联免疫吸附试验(EL ISA)微量板法。在竞争性酶联免疫吸附试验中加入底物混合改变了动态吸光度-浓度响应，大大简化了计算，提高了灵敏度和准确性。用一种市场上可买到的酶试剂盒测定尿液和血浆样品中的肌酐，其精密度和准确度与自动动力学Jaffé法相当。
We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly simplifying calculations and improving sensitivity and accuracy. Measurement of creatinine in urine and plasma samples with a commercially available enzymatic kit modified for analysis by use of an inexpensive microplate reader produced values comparable in precision and accuracy to those obtained by an automated kinetic Jaffé method.