ZNRD1 might mediate UV irradiation related DNA damage and repair in human esophageal cancer cells by regulation of ERCC1
ZNRD1 might mediate UV irradiation related DNA damage and repair in human esophageal cancer cells by regulation of ERCC1
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ZNRD1 可能通过调节 ERCC1 介导人食管癌细胞中与紫外线照射相关的 DNA 损伤和修复。
DOI：
10.1111/j.1442-2050.2008.00846.x
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Fan, D. -M.
中科院分区：
文献类型：
--
作者：
Guo, W.;Zhao, Y. -P.;Fan, D. -M.
最近发现下调含锌带结构域1（ZNRD 1）蛋白可部分逆转人白血病细胞对化学治疗药物的耐药性。因此，ZNRD 1蛋白可能参与DNA损伤和修复过程。为探讨ZNRD 1对紫外线（UV）-C辐射诱导的人食管鳞癌细胞株EC 109 DNA损伤的保护作用，设计了ZNRD 1的表达载体，转染EC 109细胞，建立了ZNRD 1过表达细胞株。用单细胞凝胶电泳（彗星试验）研究了UV-C辐射对转染细胞和对照细胞DNA的损伤和修复。发现ZNRD 1表达细胞表现出显著增强的DNA修复能力。ZNRD 1的过表达可上调ERCC 1基因的表达。这些结果提示ZNRD 1可能通过调控ERCC 1的表达在DNA损伤修复过程中发挥重要作用。
The downregulation of zinc ribbon domain-containing 1 (ZNRD1) protein was recently found to partially reverse the resistance of human leukemia cells toward chemical therapeutic drugs. Therefore, the ZNRD1 protein might be involved in the process of DNA damage and repair. To explore the possible protective effects of ZNRD1 on DNA damage induced by ultraviolet (UV)-C irradiation in human esophageal squamous cancer cell line EC109, we designed and transfected a expression vector into EC109 cells, and established an overexpression cell line. The single-cell gel electrophoresis (comet assay) was used to investigate the DNA damage and repair in UV-C-irradiated control and transfected cells. It was found that the ZNRD1-expressing cells exhibited a significant enhanced DNA repair capacity. Moreover, the overexpression of ZNRD1 could upregulate the expression of excision repair cross-complementing 1 (ERCC1) gene. Collectively, these findings suggested that ZNRD1 might play an important role in the process of DNA damage and repair by regulating the expression of ERCC1.