Oral antibiotics enhance antibody responses to keyhole limpet hemocyanin in orally but not muscularly immunized chickens
Oral antibiotics enhance antibody responses to keyhole limpet hemocyanin in orally but not muscularly immunized chickens
复制标题
口服抗生素增强口服免疫鸡对匙孔血蓝蛋白的抗体反应，但不能增强肌肉免疫鸡的抗体反应
DOI：
10.1111/asj.12424
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
F.
中科院分区：
文献类型：
--
作者：
Murai;A.;Kitahara;K.;Okumura;S.;Kobayashi;M. and Horio;F.
最近的研究强调了肠道微生物区系在触发和调节免疫反应中的关键作用。我们的目的是确定口服氨苄西林和新霉素两种抗生素对肠道微生物区系的修饰是否会导致鸡对抗原的抗体反应的变化。在饮水中分别给予或不给予氨苄西林和新霉素(0.25g/L和0.5g/L)。2周龄时，用锁孔血蓝蛋白(KLH)肌肉或口服免疫雏鸡，用双抗体夹心法检测血清中抗KLH抗体水平。在口服免疫雏鸡中，口服抗生素处理可使抗体应答(IgM、IgA、IgY)提高2~3倍，而在肌肉免疫雏鸡中，抗生素不能增强抗体应答。在增强抗体反应的同时，口服抗生素还降低了粪便中的乳杆菌种类。低剂量的抗生素(比最初的试验低10倍和100倍)未能改变粪便中乳杆菌的数量，当KLH口服免疫雏鸡时，也不会改变任何抗体反应。总之，口服抗生素治疗增强了鸡对口服暴露抗原的抗体反应。这种抗体反应的增强与粪便乳杆菌含量的改变有关，这表明肠道微生物区系与鸡的抗体反应之间可能存在联系。
Recent studies have emphasized the crucial role of gut microbiota in triggering and modulating immune response. We aimed to determine whether the modification of gut microbiota by oral co‐administration of two antibiotics, ampicillin and neomycin, would lead to changes in the antibody response to antigens in chickens. Neonatal chickens were given or not given ampicillin and neomycin (0.25 and 0.5 g/L, respectively) in drinking water. At 2 weeks of age, the chicks were muscularly or orally immunized with antigenic keyhole limpet hemocyanin (KLH), and then serum anti‐KLH antibody levels were examined by ELISA. In orally immunized chicks, oral antibiotics treatment enhanced antibody responses (IgM, IgA, IgY) by 2–3‐fold compared with the antibiotics‐free control, while the antibiotics did not enhance antibody responses in the muscularly immunized chicks. Concomitant with their enhancement of antibody responses, the oral antibiotics also lowered theLactobacillusspecies in feces. Low doses of antibiotics (10‐fold and 100‐fold lower than the initial trial), which failed to change the fecalLactobacilluspopulation, did not modify any antibody responses when chicks were orally immunized with KLH. In conclusion, oral antibiotics treatment enhanced the antibody response to orally exposed antigens in chickens. This enhancement of antibody response was associated with a modification of the fecalLactobacilluscontent, suggesting a possible link between gut microbiota and antibody response in chickens.