A Honglian CMS line of rice displays aberrant F0 of F0F1-ATPase
A Honglian CMS line of rice displays aberrant F0 of F0F1-ATPase
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DOI：
10.1007/s00299-006-0293-4
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发表时间：
2007-01
影响因子：
6.2
通讯作者：
Hong Zhang;Shaoqing Li;Ping Yi;Cuixiang Wan;Zuyu Chen;Ying-guo Zhu
中科院分区：
文献类型：
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作者：
Hong Zhang;Shaoqing Li;Ping Yi;Cuixiang Wan;Zuyu Chen;Ying-guo Zhu
红莲型是水稻三种主要的细胞质雄性不育类型之一。在以前的研究中，HL中的CMS显示与嵌合基因fH 79相关，该嵌合基因fH 79与线粒体中额外的atp 6共转录。本研究表明，HL细胞质雄性不育系完整的F0 F1-ATPase蛋白质含量和酶活性均被特异性降低，而F1区不受影响。这意味着F0部门与F1部门存在不稳定的联系。在育性恢复基因的存在下，F0 F1-ATPase可以恢复。此外，orfH 79的转录物优先被多聚腺苷酸化，并因此在恢复的杂种植株的小花中迅速降解，表明theatp 6-orfH 79参与不育表型。由于细胞色素电子传递链途径受到抑制，暗育不育株的生物量显著低于可育株。而呼吸测定结果表明，不育株的电子传递能力增强，表明不育系生物量的减少是由于F0 F1-ATPase的破坏所致。
Honglian (HL) is one of the three major types of cytoplasmic male sterility (CMS) of rice (Oryza sativaL) that has been commercially used in hybrid production. In previous studies, the CMS in HL is shown to be associated with a chimeric geneorfH79that is cotranscribed with an extra atp6 in mitochondria. This study demonstrated that the intact F0F1-ATPase in HL CMS line was specifically reduced in both of its protein quantity and enzyme activity, whereas its F1sector was not affected. It implies that the F0sector presents a labile linkage with F1. In the presence of fertility restorer gene, F0F1-ATPase can be recovered. Furthermore,orfH79transcripts were preferentially polyadenylated, and consequently degraded rapidly in florets of the restored hybrid plants, indicating that theatp6-orfH79is involved in the sterile phenotype. With inhibition of cytochrome pathway of electron transfer chain, the biomass of the sterile plants grown in dark was significantly lower than that of the fertile lines. However, the respiration measurements showed an increase in the electron transferring capacity in the sterile plants, suggesting that the reduction of biomass in sterile line was caused by the disruption of F0F1-ATPase.