Expression of a human renal sodium nucleoside cotransporter in Xenopus laevis oocytes.
Expression of a human renal sodium nucleoside cotransporter in Xenopus laevis oocytes.
复制标题
人肾钠核苷协同转运蛋白在非洲爪蟾卵母细胞中的表达。
DOI：
10.1016/0006-2952(94)00411-0
复制
发表时间：
1994
影响因子：
5.8
通讯作者：
Giacomini,KM
中科院分区：
文献类型：
--
作者：
Gutierrez,MM;Giacomini,KM
在这项研究中，非洲爪蟾laevisoocytes注射poly（A）+RNA（mRNA）分离的人肾被用来表达Na+-核苷转运蛋白。与注水的卵母细胞相比，注射50 ng poly（A）+RNA的卵母细胞对Na+刺激的[3 H]胸苷摄取增加2-3倍，注射20 ng大小分级的人肾皮质mRNA片段（2-3 kb）的卵母细胞对Na+刺激的[3 H]胸苷摄取增加4-5倍。注射2-3 kb mRNA片段的卵母细胞中的Na+依赖性胸苷摄取被胸苷和鸟苷显著抑制，但不被福霉素B抑制，这与N4 Na+-核苷协同转运蛋白一致。注射2-3 kb mRNA片段的卵母细胞Na+依赖性胸苷摄取的Km（28 μM）与人肾刷状缘膜囊泡Na+依赖性胸苷摄取的Km（27 μM）相似。这些数据首次提示人肾Na+-核苷协同转运蛋白可在X细胞中表达。光滑细胞
In this study,Xenopus laevisoocytes injected with poly(A)+RNA (mRNA) isolated from human kidney were used to express a Na+-nucleoside cotransporter. Na+-stimulated [3H]thymidine uptake was enhanced 2–3-fold in oocytes injected with 50 ng poly(A)+RNA and 4–5-fold in oocytes injected with 20 ng of a size-fractionated human renal cortex mRNA fragment (2–3 kb) in comparison with water-injected oocytes. Na+-dependent thymidine uptake in oocytes injected with the 2–3 kb mRNA fragment was inhibited significantly by thymidine and guanosine but not by formycin B, consistent with the N4 Na+-nucleoside cotransporter. TheKm(28 μM) of Na+-dependent thymidine uptake in the oocytes injected with the 2–3 kb mRNA fragment was similar to theKm(27 μM) of Na+-dependent thymidine uptake obtained in human renal brush border membrane vesicles. These data suggest for the first time that a Na+-nucleoside cotransporter from human kidney can be expressed inX. laevisoocytes.