Regulation of amylase activity in Drosophila melanogaster: Variation in the number of enzyme molecules produced by different amylase genotypes

Regulation of amylase activity in Drosophila melanogaster: Variation in the number of enzyme molecules produced by different amylase genotypes
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果蝇淀粉酶活性的调节:不同淀粉酶基因型产生的酶分子数量的变化

DOI:
10.1007/bf00484009
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发表时间:
1981
影响因子:
2.4
通讯作者:
D. Hickey
D. Hickey
中科院分区:
生物学4区
文献类型:
--
作者:
D. Hickey

文献摘要

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从高活性和低活性的黑腹果蝇变体中纯化的淀粉酶显示出相同的特异性活性。粗幼虫匀浆免疫电泳显示菌株间交叉反应物质的数量存在数倍的差异。淀粉酶活性的控制在高活性和低活性变体之间的杂合子中是“反”作用的。这些结果表明存在影响黑腹龙葵淀粉酶蛋白产生水平的多态性调控基因。
Purified amylases from high- and low-activity variants of Drosophila melanogaster showed identical specific activities. Immunoelectrophoresis of crude larval homogenates showed severalfold differences between strains in the amounts of cross-reacting material. Control of amylase activity is “trans”-acting in heterozygotes between high- and low-activity variants. These results suggest the existence of polymorphic regulatory genes affecting the production levels of amylase protein in D. melanogaster.