Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru
Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru
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DOI：
10.1086/318090
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发表时间：
2001-02-01
影响因子：
6.4
通讯作者：
Lal, AA
中科院分区：
文献类型：
--
作者：
Xiao, LH;Bern, C;Lal, AA
小隐孢子虫通常被认为是导致人类隐孢子虫病的病原体。我们使用区分隐孢子虫物种和微小隐孢子虫基因型的技术，对来自 80 名秘鲁儿童的 132 份粪便标本（代表 85 次感染事件）中的隐孢子虫进行了基因分型。鉴定出五种类型的隐孢子虫：人类隐孢子虫 (67)、牛 (8) 和狗 (2) 基因型、火鸡隐孢子虫 (7) 和猫隐孢子虫 (1)。 85 起感染事件中，有 25 起 (29%) 与腹泻有关。与人畜共患隐孢子虫相比，人类基因型的年龄、既往发育迟缓、腹泻百分比或腹泻持续时间没有显着差异。人类基因型的卵囊脱落持续时间比人畜共患隐孢子虫的卵囊脱落持续时间更长（平均分别为 13.9 天和 6.4 天；P = .004）。对 8 名携带 C. meleagridis、C. felis 或 C. parvum 犬基因型的儿童的血清样本进行了抗人类免疫缺陷病毒 (HIV) 1 型抗体检测；全部结果均为阴性。与普遍看法相反，新的隐孢子虫物种和微小隐孢子虫基因型可以感染艾滋病毒阴性儿童。
Cryptosporidium parvum is usually considered to be the pathogen responsible for human cryptosporidiosis. We genotyped Cryptosporidium in 132 stool specimens from 80 Peruvian children, representing 85 infection episodes, using techniques that differentiate Cryptosporidium species and C. parvum genotypes. Five types of Cryptosporidium were identified: C. parvum human (67), bovine (8), and dog (2) genotypes, C. meleagridis (7), and C. felis (1). Twenty-five (29%) of the 85 infection episodes were associated with diarrhea. There was no significant difference in age, antecedent stunting, percentage with diarrhea, or duration of diarrhea for episodes with human genotype, compared with those of zoonotic Cryptosporidium. Duration of oocyst shedding was longer for human genotype than for zoonotic Cryptosporidium (mean, 13.9 days and 6.4 days, respectively; P = .004). Serum samples from 8 children with C. meleagridis, C. felis, or C. parvum dog genotype were tested for anti-human immunodeficiency virus (HIV) type 1 antibodies; all were found to be negative. Contrary to common belief, novel Cryptosporidium species and C. parvum genotypes can infect HIV-negative children.