ADAPTATION OF COENZYME STIMULATION ASSAYS FOR THE NUTRITIONAL ASSESSMENT OF VITAMIN-B1, VITAMIN-B2, VITAMIN-B6 USING THE COBAS BIO CENTRIFUGAL ANALYZER
ADAPTATION OF COENZYME STIMULATION ASSAYS FOR THE NUTRITIONAL ASSESSMENT OF VITAMIN-B1, VITAMIN-B2, VITAMIN-B6 USING THE COBAS BIO CENTRIFUGAL ANALYZER
复制标题
DOI：
10.1177/000456328702400106
复制
发表时间：
1987-01-01
影响因子：
2.2
通讯作者：
MARSH, A
中科院分区：
文献类型：
--
作者：
MOUNT, JN;HEDUAN, E;MARSH, A
本文介绍了在Cobas Bio离心分析仪上对维生素B1、B2和B6进行辅酶刺激测定的适应性。在测定前，将全血收集到酸-柠檬酸葡萄糖中，其保存红细胞数天。洗涤后的红细胞储存在-70 ℃C和随后解冻，显示改变的酶活性。该方法提供了比现有程序更高的精度，并利用了仪器的高通量能力。
Adaptation of coenzyme stimulation assays for the nutritional assessment of thiamin, riboflavin and pyridoxine on the Cobas Bio centrifugal analyser are described. Whole blood was collected into acid-citrate dextrose, which preserves the erythrocytes, prior to assay for several days. Washed erythrocytes stored at -70.degree. C and subsequently thawed, showed altered enzyme activities. The methods offer improved precision over existing procedures and take advantage of the high throughout capabilities of the instrumentation.