Antioxidant responses and gene expression in perennial ryegrass (Lolium perenne L.) under cadmium stress
Antioxidant responses and gene expression in perennial ryegrass (Lolium perenne L.) under cadmium stress
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DOI：
10.1007/s10646-011-0628-y
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发表时间：
2011-06-01
期刊：
影响因子：
2.7
通讯作者：
Fu, Jinmin
中科院分区：
文献类型：
--
作者：
Luo, Hongji;Li, Huiying;Fu, Jinmin
多年生黑麦草(Lolium perenne L.)是一种富镉(Cd)的草坪草，广泛应用于温带气候。本研究旨在探讨多年生黑麦草对Cd胁迫的生理响应和候选基因表达。在温室内，将牧草置于0、0.2和0.5 mM Cd三个水平下，共处理7天。结果表明，Cd处理的多年生黑麦草的可溶性蛋白质含量低于未处理的黑麦草。与对照相比，Cd处理的多年生黑麦草表现出更高的丙二醛水平和过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活性。Cd胁迫诱导多年生黑麦草在处理后4~24 h FeSOD、MnSOD、Chl-Cu/ZnSOD、Cyt-Cu/ZnSOD、APX、GPX、GR和POD的表达上调。结果表明，基因转录谱与Cd胁迫下的酶活性有关。
Perennial ryegrass (Lolium perenne L.), widely used in temperate climates, is one of turf grasses that enrich cadmium (Cd). The objective of this study was to explore the physiological responses and candidate gene expression in perennial ryegrass to Cd stress. Grasses were subjected to three levels of 0, 0.2, and 0.5 mM Cd for 7 days in the greenhouse. The results indicated that soluble protein content was lower in the Cd-treated perennial ryegrass compared to untreated plants. The Cd-treated perennial ryegrass exhibited a greater level of malondialdehyde and activity of the peroxidase (POD), catalase, and superoxide dismutase (SOD) relative to the control. The Cd stress induced up-regulated expression of FeSOD, MnSOD, Chl Cu/ZnSOD, Cyt Cu/ZnSOD, APX, GPX, GR and POD at 4-24 h after treatment began for perennial ryegrass. Results suggested that the gene transcript profile was related to the enzyme activity under Cd stress.