Carbon nanotube electrodes for retinal implants: A study of structural and functional integration over time.
Carbon nanotube electrodes for retinal implants: A study of structural and functional integration over time.
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DOI：
10.1016/j.biomaterials.2016.10.018
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发表时间：
2017-01
期刊：
影响因子：
14
通讯作者：
Sernagor E
中科院分区：
文献类型：
--
作者：
Eleftheriou CG;Zimmermann JB;Kjeldsen HD;David-Pur M;Hanein Y;Sernagor E
电极材料的选择在神经假体装置中是至关重要的。电极必须是生物相容的，但能够在高度腐蚀性的环境中维持重复的电流注入。我们探讨了碳纳米管（CNT）电极刺激视网膜神经节细胞（RGC）在小鼠模型的外部视网膜变性的适用性。我们研究了生物混合界面的形态变化和RGC对电刺激的响应变化，这些变化是在体外长时间耦合到CNT电极后发生的。我们观察到逐渐重塑的视网膜内层纳入CNT组件。电生理记录表明，在三天内，RGCs和CNT电极之间的耦合逐渐增加，其特征在于刺激阈值逐渐降低，细胞募集增加。这些结果为CNT和视网膜之间的可行生物混合物的时间依赖性形成提供了新的证据，表明CNT是用于包含在视网膜假体装置中的有前途的材料。
The choice of electrode material is of paramount importance in neural prosthetic devices. Electrodes must be biocompatible yet able to sustain repetitive current injections in a highly corrosive environment. We explored the suitability of carbon nanotube (CNT) electrodes to stimulate retinal ganglion cells (RGCs) in a mouse model of outer retinal degeneration. We investigated morphological changes at the bio-hybrid interface and changes in RGC responses to electrical stimulation following prolonged in vitro coupling to CNT electrodes. We observed gradual remodelling of the inner retina to incorporate CNT assemblies. Electrophysiological recordings demonstrate a progressive increase in coupling between RGCs and the CNT electrodes over three days, characterized by a gradual decrease in stimulation thresholds and increase in cellular recruitment. These results provide novel evidence for time-dependent formation of viable bio-hybrids between CNTs and the retina, demonstrating that CNTs are a promising material for inclusion in retinal prosthetic devices.