Burkholderia ambifaria sp nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates
Burkholderia ambifaria sp nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates
复制标题
DOI：
10.1099/00207713-51-4-1481
复制
发表时间：
2001-07-01
影响因子：
2.8
通讯作者：
Vandamme, P
中科院分区：
文献类型：
--
作者：
Coenye, T;Mahenthiralingam, E;Vandamme, P
对19株洋葱伯克霍尔德菌进行了多相分类研究，包括扩增片段长度多态性(AFLP)指纹图谱、DNA-DNA杂交、DNA碱基比测定、系统发育分析、全细胞脂肪酸分析和广泛的生化特征。一些环境分离株因其生防特性而引起了人们的极大兴趣。多相分类数据表明，这些菌株代表洋葱假单胞菌复合体的一个新成员，命名为Burkholderia ambiaria sp.Nov.，提出了。模式菌株为菌株1MG 19182(T)。通过AFLP指纹图谱、全细胞脂肪酸分析、生化试验(包括鸟氨酸和赖氨酸脱羧酶活性、蔗糖酸化和P-溶血)和最新建立的基于recA基因的聚合酶链式反应分析，16S rDNA的RFLP分析和聚合酶链式反应可以将两栖芽孢杆菌与洋葱伯克霍尔德氏菌复合体的其他成员区分开来，但不能将其与洋葱伯克霍尔德氏菌、伯克霍尔德里亚vietnamiensis和B、洋葱基因组VI区分开来。这个新的分类群既包括从CF患者分离的菌株，也包括潜在有用的生防菌株，这一发现支持了普遍的共识，即在更多地了解其潜在的致病机制之前，大规模使用属于洋葱假单胞菌复合体的生防菌株是不明智的。
A polyphasic taxonomic study, including amplified fragment length polymorphism (AFLP) fingerprinting, DNA-DNA hybridizations, DNA base-ratio determinations, phylogenetic analysis, whole-cell fatty acid analyses and an extensive biochemical characterization, was performed on 19 Burkholderia cepacia-like isolates from the environment and cystic fibrosis (CF) patients. Several of the environmental isolates have attracted considerable interest due to their biocontrol properties. The polyphasic taxonomic data showed that the strains represent a new member of the B. cepacia complex, for which the name Burkholderia ambifaria sp. nov, is proposed. The type strain is strain LMG 19182(T). B, ambifaria can be differentiated from the other members of the B. cepacia complex by means of AFLP fingerprinting, whole-cell fatty acid analysis, biochemical tests (including ornithine and lysine decarboxylase activity, acidification of sucrose and P-haemolysis) and a newly developed recA gene-based PCR assay, 16S rDNA-based RFLP analysis and PCR tests allowed differentiation of B. ambifaria from Burkholderia multivorans, Burkholderia vietnamiensis and B, cepacia genomovar VI, but not from B. cepacia genomovars I and III and Burkholderia stabilis. The finding that this new taxon includes both strains isolated from CF patients and potentially useful biocontrol strains supports the general consensus that the large-scale use of biocontrol strains belonging to the B. cepacia complex would be ill-advised until more is known about their potential pathogenic mechanisms.