c-fos mRNA Induction in Acute and Chronic Audiogenic Stress: Possible Role of the Orbitofrontal Cortex in Habituation
c-fos mRNA Induction in Acute and Chronic Audiogenic Stress: Possible Role of the Orbitofrontal Cortex in Habituation
复制标题
DOI：
10.1080/10253890290027895
复制
发表时间：
2002-01-01
影响因子：
2.3
通讯作者：
Watson, Stanley J., Jr.
中科院分区：
文献类型：
--
作者：
Campeau, Serge;Dolan, David;Watson, Stanley J., Jr.
为了研究假定的涉及对压力的习惯化的脑回路，将大鼠每天（30分钟，持续15天）暴露于存在（慢性）或不存在（急性）大声噪声（105 dB声压级-SPL A量表）的环境中。行为和内分泌措施的压力，在整个适应期，这两个措施显示出强烈的习惯在慢性组。所有大鼠在第16天暴露后立即处死，构成急性组的急性应激源，并使用原位杂交的c-fos mRNA诱导评估局部脑活动。听力损伤不能轻易解释这些结果，因为暴露于类似应激方案的其他大鼠的听觉脑干诱发电位没有变化。急性组和慢性组的中枢听觉系统中c-fos mRNA的诱导相似，特别是在较低的听觉处理水平，也反对慢性应激大鼠听觉处理的简单减少。然而，c-fos mRNA的表达减少慢性，急性，应激大鼠在几个地区以前牵连在听源性应激（外侧隔，床核的终纹，一些视前区，和室旁下丘脑核）。有趣的是，眶额皮质是唯一的区域显示较高的c-fos mRNA的诱导，在慢性相比，急性应激大鼠。该区域与几个应激反应区域有联系，因此可能是积极抑制应激的关键区域。
To study putative brain circuits involved in habituation to stress, rats were exposed daily (30 min for 15 days) to an environment in the presence (Chronic) or absence (Acute) of loud noise (105 dB sound pressure level-SPL A Scale). Behavioral and endocrine measures of stress were taken throughout this habituation period, and both measures displayed strong habituation in the Chronic group. All rats were killed immediately after the day 16 exposure, constituting an acute stressor for the Acute group, and regional brain activity was assessed using c-fos mRNA induction with in situ hybridization. Hearing damage could not easily explain these results because additional rats exposed to a similar stress protocol exhibited no changes in auditory brainstem evoked potentials. c-fos mRNA induction in the central auditory system was similar between the Acute and Chronic groups, particularly at lower auditory processing levels, also arguing against a simple reduction in auditory processing in the chronically stressed rats. However, c-fos mRNA expression was reduced in chronically, as compared to acutely, stressed rats in several regions previously implicated in audiogenic stress (lateral septum, bed nucleus of the stria terminalis, some preoptic areas, and the paraventricular hypothalamic nucleus). Interestingly, the orbitofrontal cortex was the only region displaying higher c-fos mRNA induction in the chronically as compared to acutely stressed rats. This region has connections to several stress-responsive areas and may thus be a critical region actively inhibiting stress.