Analysis of differentially expressed circular RNAs in endothelial cells under impinging flow
Analysis of differentially expressed circular RNAs in endothelial cells under impinging flow
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冲击流下内皮细胞差异表达环状RNA分析
DOI：
10.1016/j.mcp.2020.101539
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发表时间：
2020-06-01
影响因子：
3.3
通讯作者：
Li, Meihua
中科院分区：
文献类型：
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作者：
Hao, Zheng;Li, Yiyun;Li, Meihua
背景：环状RNA(CircRNAs)是一种特殊的非编码RNA。为了阐明血流动力学与内皮细胞CircRNAs功能的关系，为本实验设计和制造了一种改进的T室系统。方法：将培养于玻片上的正常内皮细胞置于T小室内，以500mL/min的流速冲击内皮细胞层，用高通量芯片分析内皮细胞CircRNA的表达谱。比较了撞击流处理的内皮细胞和常规培养条件下的内皮细胞CircRNAs的差异表达。利用生物信息学方法对差异表达的CircRNA进行特征分析，并通过定量逆转录聚合酶链式反应对结果进行验证。结果：与正常组织相比，许多CircRNA的表达发生了变化。共有974个CircRNA差异表达，其中378个上调，596个下调(折叠式变化[Fc]>；=2，P<；0.05)，这表明这些CircRNA在血流动力学条件下发生了变化。结论：我们给出了撞击流作用后内皮细胞CircRNAs的差异表达谱，我们的结果表明这些差异表达的CircRNAs可能参与了内皮细胞的炎症反应和损伤。目前的发现提供了有价值的关于cRNA图谱的信息以及未来研究的线索，这些研究将调查CircRNAs在炎症损伤后内皮细胞中所起的作用。
Background: Circular RNAs (circRNAs) are a special type of non-coding RNA. To elucidate the relationship between hemodynamics and the function of circRNAs in endothelial cells (ECs), a modified T chamber system was designed and produced for the present experiment. This T chamber system can be used to simulate the hemodynamic environment at the bifurcation of the arteries.Methods: Normal ECs cultured on glass slides were placed in the T chamber, the cell layer was impacted at a flow rate of 500 mL/min, and high-throughput microarrays were used to analyze the expression profiles of circRNAs in ECs. The differential expressions of circRNAs in the ECs treated with impinging flow were compared to those in ECs in conventional culture conditions. The characteristics of the differentially expressed circRNAs were analyzed with bioinformatics and quantitative reverse transcription polymerase chain reaction analyses were conducted to verify results.Results: Compared to normal samples, there were changes in the expressions of many circRNAs. A total of 974 circRNAs were differentially expressed, and of these, 378 were upregulated and 596 were downregulated (fold change [FC] >= 2 and P < 0.05), which suggests that these circRNAs were altered under hemodynamic conditions.Conclusions: We present the differential expression profiles of circRNAs in ECs after the application of impinging flow; our results indicate that these differentially expressed circRNAs may be involved in inflammatory responses and damage in ECs. The present findings provide valuable information on cRNA profiles as well as clues for future studies that will investigate the roles that circRNAs play in ECs after inflammatory injury.