Ovarian and hormonal responses to a progesterone-releasing controlled internal drug releasing treatment in dietary-restricted goats
Ovarian and hormonal responses to a progesterone-releasing controlled internal drug releasing treatment in dietary-restricted goats
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DOI：
10.1016/j.anireprosci.2003.12.007
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发表时间：
2004-08-01
影响因子：
2.2
通讯作者：
Kaneda, Y
中科院分区：
文献类型：
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作者：
Tanaka, T;Fujiwara, K;Kaneda, Y
本试验旨在评价限饲对经炔雌醇释放体内药物控释装置（CIDR-G）处理的山羊卵巢、内分泌（卵巢类固醇和促黄体生成激素（LH）脉冲）和代谢（葡萄糖、胰岛素和非脂肪酸（NEFA））特征的影响。骑自行车的山羊提供维持或限制（30%的要求; n = 4每治疗）的喂养水平。在排卵后的第二天开始饮食限制。开始限食后30-32天，山羊接受前列腺素F-2 α（2 mg地诺前列素）注射，随后接受10天CIDR-G治疗。在整个实验过程中，每天监测卵巢超声图像，并在每天早晨喂食前收集血液样品用于内分泌和代谢谱的分析。从-8小时到CIDR-G去除，以及从CIDR-G去除后32小时到40小时，以10分钟的间隔收集频繁的血液样品（1 ml），持续8小时，用于LH脉冲分析。限食组动物体重显著下降（P < 0.05）。在所有对照动物中，在去除CIDR-G后12天内观察到发情行为和排卵，随后血浆孕酮浓度升高，但在任何限食动物中均未观察到。在去除CIDR-G后32至40小时，食物限制动物的LH脉冲频率显著（P < 0.05）低于对照动物（1.5 +/- 0.6 vs 3.8 +/- 0.5脉冲，8小时）。对照组和限食组动物每周血浆样本中的葡萄糖浓度无显著差异。从开始限饲后2周开始，限饲动物的胰岛素浓度显著（P < 0.05）低于对照动物。限饲组动物的NEFA浓度在开始限饲后显著升高（P < 0.05），随后逐渐下降至基础水平。目前的结果表明，CIDR-G治疗后营养诱导的无排卵与LH脉冲的频率降低有关，胰岛素和NEFA，而不是循环中的葡萄糖浓度，可能与LH脉冲的代谢抑制有关。(C)2004 Elsevier B. V.保留所有权利。
An experiment was conducted to evaluate the effects of dietary restriction on ovarian, endocrine (ovarian steroids and luteinizing hormone (LH) pulse) and metabolic (glucose, insulin and non-esteritied fatty acid (NEFA)) profiles in goats treated with a progesterone-releasing controlled internal drug releasing (CIDR-G) device. Cycling goats were offered either a maintenance or a restricted (30% of requirement; n = 4 per treatment) level of feeding. The dietary restriction was started on the day following ovulation. At 30-32 days after the start of food restriction, the goats received a prostaglandin F-2alpha (2 mg of dinoprost) injection followed by 10 days of CIDR-G treatment. Ovarian ultrasonographic images were monitored daily throughout the experiment and blood samples were collected daily just before the morning feeding for analysis of endocrine and metabolic profiles. Frequent blood samples (1 ml) were also collected at 10 min intervals for 8 h from - 8 h to CIDR-G removal, and from 32 to 40 h after CIDR-G removal for analysis of LH pulses. Body weight was significantly (P < 0.05) decreased in the food-restricted animals. Oestrous behaviour and ovulation followed by a rise of plasma progesterone concentration were observed after the CIDR-G removal in all control animals but not in any of the food-restricted animals within 12 days after CIDR-G removal. The LH pulse frequency from 32 to 40 h after the CIDR-G removal was significantly (P < 0.05) lower in the food-restricted animals than in control animals (1.5 +/- 0.6 versus 3.8 +/- 0.5 pulses for 8 h). There was no significant difference in the glucose concentration in weekly plasma samples between control and food-restricted animals. Insulin concentrations from 2 weeks after the start of feed restriction were significantly (P < 0.05) lower in restricted animals than in control animals. The NEFA concentration in restricted animals was significantly (P < 0.05) increased after the start of feed restriction, and then decreased gradually to the basal level. The present results suggest that nutritionally induced anovulation after CIDR-G treatment is associated with a reduction in the frequency of LH pulses, and that insulin and NEFA, rather than the glucose concentration in the circulation, may be associated with the metabolic suppression of LH pulses. (C) 2004 Elsevier B.V. All rights reserved.