KINETIC MECHANISM OF HALOBACTERIUM-HALOBIUM NAD+-GLUTAMATE DEHYDROGENASE
KINETIC MECHANISM OF HALOBACTERIUM-HALOBIUM NAD+-GLUTAMATE DEHYDROGENASE
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DOI：
10.1016/s0304-4165(89)80027-3
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发表时间：
1989-02-24
期刊：
影响因子：
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通讯作者：
CADENAS, E
中科院分区：
文献类型：
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作者：
BONETE, MJ;CAMACHO, ML;CADENAS, E
研究了盐生盐杆菌NAD+-谷氨酸脱氢酶(EC 1.4.1.3)在pH 9.0、3M NaC l和40℃下的动力学机制。C在两个方向上，通过初始速率和抑制研究。初始速率研究的结果表明，与底物添加有关的机理是连续的。未经修饰的嗜盐NAD+-谷氨酸脱氢酶的抑制模式不符合简单的有序机制。然而，通过假设一个适当的流产情结，它们可以与这种类型的机制相协调。
The kinetic mechanism of Halobacterium halobium NAD+-glutamate dehydrogenase (EC 1.4.1.3) has been investigated at pH 9.0, 3 M NaCl and 40.degree. C in both directions, by initial rate and inhibition studies. The results of the initial rate studies indicate that the mechanism is sequential with respect to substrate addition. The inhibition patterns obtained with halophilic NAD+-glutamate dehydrogenase are not consistent with a simple ordered mechanism without modification. They can, however, be reconciled with this type of mechanism by postulating an appropriate abortive complex.