ACTION SPECTRA OF PHYTOCHROME INVITRO
ACTION SPECTRA OF PHYTOCHROME INVITRO
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DOI：
10.1111/j.1751-1097.1964.tb08171.x
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发表时间：
1964-01-01
影响因子：
3.3
通讯作者：
SIEGELMAN, HW
中科院分区：
文献类型：
--
作者：
BUTLER, WL;HENDRICKS, SB;SIEGELMAN, HW
在300至800 nm之间的各种波长下测量光敏色素PR和PFR两种形式的光化学转化的一级速率常数Kλ。在任何波长λ下，消光系数ε和量子产率ε的乘积可以由Kλ值和λ设定的光稳态下PR和PVR的摩尔分数确定。确定作用（ε ν）光谱需要知道红光和远红光中的光稳态。这些是由吸收光谱和动力学数据确定的。
First‐order rate constants, Kλ, for the photochemical conversions of both forms of phytochrome, PRand PFR, were measured at various wavelengths between 300 and 800 nm. The product of the extinction coefficient, ε, and the quantum yield, ø, at any wave‐length, λ, could be determined from the value of Kλand the mole fractions of PRand PFRpresent at the photostationary state set by λ. The determination of the action (εø) spectra required that the photostationary states in red and far‐red light be known. These were determined from the absorption spectra and kinetic data.