Elevation of granulocyte-macrophage colony-stimulating factor in the placenta and blood in preeclampsia
Elevation of granulocyte-macrophage colony-stimulating factor in the placenta and blood in preeclampsia
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DOI：
10.1016/j.ajog.2003.07.032
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发表时间：
2004-02-01
影响因子：
9.8
通讯作者：
Ohkura, T
中科院分区：
文献类型：
--
作者：
Hayashi, M;Hamada, Y;Ohkura, T
目的：本研究调查了先兆子痫患者胎盘和血液中粒细胞-巨噬细胞集落刺激因子（GM-CSF）水平是否与正常妊娠不同。胎盘中的巨噬细胞集落刺激因子（M-CSF）水平也measured.Study设计：受试者是44名孕妇携带单胎，其中22名妇女正常妊娠和22名妇女先兆子痫。她们的平均孕龄为37至38周。在分娩开始前采集外周血。离心后获得分离的血清，并在-20 ℃下储存。分娩后立即切除胎盘的一个组织段。将冷冻的胎盘组织置于含有磷酸盐缓冲盐水溶液的塑料管中。将组织完全均质化，然后离心。将分离的上清液在-80 ℃下冷冻用于随后的测定。结果：胎盘组织中GM-CSF、GM-CSF/TP比值均显著高于正常对照组（P < 0.05），胎盘组织中GM-CSF、M-CSF、TP比值均显著高于正常对照组（P < 0.05），胎盘组织中GM-CSF、M-CSF、TP比值均显著高于正常对照组（P < 0.05）。
Objective: This study investigated whether granulocyte-macrophage colony-stimulating factor (GM-CSF) levels in the placenta and blood in preeclampsia differed from those in normal pregnancies. Macrophage colony-stimulating factor (M-CSF) levels in the placenta were also measured.Study design: The subjects were 44 pregnant women carrying single fetuses, of whom 22 were women with normal pregnancies and 22 were women with preeclampsia. Their average gestational age at entry was 37 to 38 weeks of gestation. Peripheral blood was collected before the onset of labor. Separated serum was obtained after centrifugation and stored at -20degreesC. A tissue segment of the placenta was cut immediately after delivery. The frozen placental tissue was placed into a plastic tube containing phosphate-buffered saline solution. The tissue was fully homogenized and then centrifuged. Separated supernatant was frozen at -80degreesC for subsequent determination. GM-CSF levels in separated serum were measured, and GM-CSF, M-CSF, and total protein (TP) levels in separated supernatant were also measured.Results: Not only GM-CSF levels in blood but also GM-CSF/TP levels in the placenta were significantly higher (P