Mitochondria in human offspring derived from ooplasmic transplantation
Mitochondria in human offspring derived from ooplasmic transplantation
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DOI：
10.1093/humrep/16.3.513
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发表时间：
2001-03-01
影响因子：
6.1
通讯作者：
Cohen, J
中科院分区：
文献类型：
--
作者：
Barritt, JA;Brenner, CA;Cohen, J
从可育的供体卵母细胞到潜在受损的受体患者卵母细胞的卵质转移已经导致全世界近30个婴儿的出生。细胞质移植引起了人们的担忧，因为来自两个不同母体的人类卵质的混合可能会在后代中产生线粒体（mt）异质性（受体和供体mtDNA）。这项调查追踪线粒体供体人口在卵浆转移技术和两个1岁的儿童使用mtDNA指纹。供体卵浆染色的活性线粒体转移到受体卵浆中，并在显微注射程序和受精后通过共聚焦显微镜观察线粒体。在每个孩子的血液中都发现了异质性。这份报告是人类生殖系基因改造导致正常健康儿童的首例。
Ooplasmic transfer from fertile donor oocytes into potentially compromised recipient patient oocytes has led to the birth of nearly 30 babies worldwide. Cytoplasmic transplantation has caused apprehension, since the mixing of human ooplasm from two different maternal sources may generate mitochondrial (mt) heteroplasmy (both recipient and donor mtDNA) in offspring. This investigation traced the mitochondrial donor population both during the ooplasmic transfer technique and in the bloods of two 1 year old children using mtDNA fingerprinting. Donor ooplasm stained for active mitochondria was transferred into recipient ooplasm and the mitochondria were visualized by confocal microscopy after the microinjection procedure and fertilization. Heteroplasmy was found in the blood from each of the children. This report is the first case of human germline genetic modification resulting in normal healthy children.