Klf5 is involved in self-renewal of mouse embryonic stem cells
Klf5 is involved in self-renewal of mouse embryonic stem cells
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DOI：
10.1242/jcs.027599
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发表时间：
2008-08-15
影响因子：
4
通讯作者：
Russo, Tommaso
中科院分区：
文献类型：
--
作者：
Parisi, Silvia;Passaro, Fabiana;Russo, Tommaso
胚胎干细胞（ESCs）的自我更新是由一个复杂的调控机制维持的，涉及转录因子Oct 3/4（Pou 5 f1），Nanog和Sox 2。在这里，我们报告Klf 5，锌指转录因子的Kruppel样家庭，参与ESC自我更新。Klf 5在小鼠胚胎干细胞、胚泡和原始生殖细胞中表达，通过RNA干扰敲低Klf 5可改变胚胎干细胞的分子表型，从而阻止其正确分化。Klf 5维持ESC处于未分化状态的能力得到以下发现的支持：当Klf 5组成型表达时，ESC的分化被阻止。Klf 5维持未分化状态至少部分是由于Nanog和Oct 3/4转录的控制，因为Klf 5直接结合这些基因的启动子并调节它们的转录。
Self-renewal of embryonic stem cells (ESCs) is maintained by a complex regulatory mechanism involving transcription factors Oct3/4 (Pou5f1), Nanog and Sox2. Here, we report that Klf5, a Zn-finger transcription factor of the Kruppel-like family, is involved in ESC self-renewal. Klf5 is expressed in mouse ESCs, blastocysts and primordial germ cells, and its knockdown by RNA interference alters the molecular phenotype of ESCs, thereby preventing their correct differentiation. The ability of Klf5 to maintain ESCs in the undifferentiated state is supported by the finding that differentiation of ESCs is prevented when Klf5 is constitutively expressed. Maintenance of the undifferentiated state by Klf5 is, at least in part, due to the control of Nanog and Oct3/4 transcription, because Klf5 directly binds to the promoters of these genes and regulates their transcription.