Chemical comparison of goldenseal (Hydrastis canadensis L.) root powder from three commercial suppliers.
Chemical comparison of goldenseal (Hydrastis canadensis L.) root powder from three commercial suppliers.
复制标题
DOI：
10.1021/jf034339r
复制
发表时间：
2003-11
影响因子：
6.1
通讯作者：
H. A. Weber;M. K. Zart;A. E. Hodges;H. Molloy;B. O'brien;Leslie A Moody;Alice P Clark;R. K. Harris;J. Overstreet;Cynthia S. Smith
中科院分区：
文献类型：
--
作者：
H. A. Weber;M. K. Zart;A. E. Hodges;H. Molloy;B. O'brien;Leslie A Moody;Alice P Clark;R. K. Harris;J. Overstreet;Cynthia S. Smith
草药材料的表征是分析化学家面临的一个重大挑战。白毛茛（Hydrastis canadensis L.），已被NIEHS选择进行毒性评估，是目前市场上排名前15位的草药补充剂之一，含有从碳水化合物和氨基酸到异喹啉生物碱的复杂混合物。草药补充剂生产的一个关键组成部分是植物认证，这也是在开始功效或毒理学研究之前建议的。为了评估消费者可用的材料，从三个商业供应商获得了白毛茛根粉，并制定了一项表征和比较策略，包括索氏提取、HPLC、GC-MS和LC-MS分析。采用高效液相色谱法测定各种提取物残留物中的黄连生物碱小檗碱、海斯汀和坎那丁的重量百分比。巴马汀是一种异喹啉生物碱，原产于黄连属。和其它常见的毛茛混淆品，也用HPLC进行了定量。采用气相色谱-质谱联用技术对黄精粉中非生物碱成分进行鉴定，采用液相色谱-质谱联用技术对生物碱成分进行鉴定。在审查表征数据后，确定生物碱含量是毛茛的最佳生物标志物。还开发了20 min环境提取法测定生物碱含量，并用于分析商业材料。所有三个采购批次的材料均含有毛茛生物碱海斯汀、小檗碱、四氢小檗碱、加拿大碱、小檗碱、海斯汀和加拿大碱。来自单一供应商的材料还含有巴马汀、黄连碱和药根碱，因此表明该材料不是纯的毛茛。三个商业来源的黄精根粉的比较数据。
The characterization of herbal materials is a significant challenge to analytical chemists. Goldenseal (Hydrastis canadensis L.), which has been chosen for toxicity evaluation by NIEHS, is among the top 15 herbal supplements currently on the market and contains a complex mixture of indigenous components ranging from carbohydrates and amino acids to isoquinoline alkaloids. One key component of herbal supplement production is botanical authentication, which is also recommended prior to initiation of efficacy or toxicological studies. To evaluate material available to consumers, goldenseal root powder was obtained from three commercial suppliers and a strategy was developed for characterization and comparison that included Soxhlet extraction, HPLC, GC-MS, and LC-MS analyses. HPLC was used to determine the weight percentages of the goldenseal alkaloids berberine, hydrastine, and canadine in the various extract residues. Palmatine, an isoquinoline alkaloid native to Coptis spp. and other common goldenseal adulterants, was also quantitated using HPLC. GC-MS was used to identify non-alkaloid constituents in goldenseal root powder, whereas LC-MS was used to identify alkaloid components. After review of the characterization data, it was determined that alkaloid content was the best biomarker for goldenseal. A 20-min ambient extraction method for the determination of alkaloid content was also developed and used to analyze the commercial material. All three lots of purchased material contained goldenseal alkaloids hydrastinine, berberastine, tetrahydroberberastine, canadaline, berberine, hydrastine, and canadine. Material from a single supplier also contained palmatine, coptisine, and jatrorrhizine, thus indicating that the material was not pure goldenseal. Comparative data for three commercial sources of goldenseal root powder are presented.