Endothelial Cell Vascular Smooth Muscle Cell Co-Culture Assay For High Throughput Screening Assays For Discovery of Anti-Angiogenesis Agents and Other Therapeutic Molecules.
Endothelial Cell Vascular Smooth Muscle Cell Co-Culture Assay For High Throughput Screening Assays For Discovery of Anti-Angiogenesis Agents and Other Therapeutic Molecules.
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DOI：
10.2147/ijhts.s13459
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发表时间：
2010-10-01
期刊：
影响因子：
--
通讯作者：
Truskey GA
中科院分区：
文献类型：
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作者：
Truskey GA
复制体内生理学关键要素的精确体外模型系统将极大地帮助许多疾病的药物开发。最近开发的内皮细胞和血管平滑肌细胞共培养系统可以扩展到高通量系统，用于鉴定血管生成、血管修复和高血压的化合物。在这篇综述中，回顾了各种共培养系统，并讨论了内皮细胞和血管平滑肌细胞之间的生物相互作用。介绍了高通量系统设计中的关键考虑因素，并讨论了选定的示例。
Drug development for many diseases would be aided greatly by accurate in vitro model systems that replicate key elements of in vivo physiology. The recent development of co-culture systems of endothelial cells and vascular smooth muscle cells can be extended to high throughput systems for the identification of compounds for angiogenesis, vascular repair and hypertension. In this review, the various co-culture systems are reviewed and biological interactions between endothelial cells and vascular smooth muscle cells are discussed. Key considerations in the design of high throughput systems are presented and selected examples are discussed.