Effects of warm acclimation on Na+,K+-ATPase alpha-subunit expression in chloride cells of Antarctic fish.

Effects of warm acclimation on Na+,K+-ATPase alpha-subunit expression in chloride cells of Antarctic fish.
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温驯化对南极鱼氯细胞Na,K-ATP酶α亚基表达的影响。

DOI:
10.1002/ar.a.20203
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发表时间:
2005
期刊:
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
影响因子:
--
通讯作者:
D. Petzel
D. Petzel
中科院分区:
--
文献类型:
--
作者:
P. Brauer;J. Sanmann;D. Petzel

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硬骨鱼Trematomus bernacchii在温度低于0摄氏度的南部海洋沃茨中茁壮成长。这些鱼类的血清渗透压几乎是温带沃茨鱼类的两倍,从而降低了它们血清的冰点和离子稳态所需的能量。在温暖适应至4摄氏度后,T.降低血清渗透压,提高鳃Na+,K+-ATP酶活性。Na+,K+-ATP酶α 1-、α 2-和α 3-亚基在T. bernacchii的Na ~+,K ~+-ATPase亚基组成随温驯化而变化。利用免疫组织化学方法,我们比较了冷驯化和热驯化的红腹锦鸡儿鳃中表达Na+,K+-ATP酶各种α亚型的氯细胞数量。bernacchii。我们发现,在α 2-或α 3-免疫阳性细胞的数量在温暖的驯化鱼鳃或细胞的Na+,K+,2Cl-协同转运蛋白的免疫阳性的数量没有变化。然而,泛α-免疫阳性(识别所有三个α-亚型)和α 1-免疫阳性细胞的数量增加,在温暖的驯化鱼。这表明,α 1-异构体表达氯细胞的数量的变化可能有助于增加的Na+,K+-ATP酶活性,发生与热驯化。
The teleosts Trematomus bernacchii thrive in southern oceanic waters with temperatures below 0 degrees C. These fish have serum osmolalities almost double those found in fish of temperate waters, thereby lowering their serum's freezing point and the energy needed for ionic homeostasis. Upon warm acclimation to 4 degrees C, T. bernacchii decrease their serum osmolality and increase the Na+,K+-ATPase activity in their gills. Na+,K+-ATPase alpha1-, alpha2-, and alpha3-subunit isoforms are expressed in the gills of T. bernacchii and it is thought that Na+,K+-ATPase subunit composition in chloride cells changes with warm acclimation. Using immunohistochemistry, we compared the number of chloride cells expressing various alpha-isoforms of the Na+,K+-ATPase in the gills of cold- and warm-acclimated T. bernacchii. We found no change in the number of alpha2- or alpha3-immunopositive cells in warm-acclimated fish gills or in the number of cells immunopositive for the Na+,K+,2Cl- cotransporter. However, the number of pan-alpha-immunopositive (recognizing all three alpha-isoforms) and alpha1-immunopositive cells both increased in warm-acclimated fish. This suggests that changes in the number of alpha1-isoform-expressing chloride cells could contribute to the increased Na+,K+-ATPase activity that occurs with warm-acclimation.
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