GYNOGENESIS IN COMMON CARP (CYPRINUS-CARPIO L) .2. THE PRODUCTION OF HOMOZYGOUS GYNOGENETIC CLONES AND F1 HYBRIDS
GYNOGENESIS IN COMMON CARP (CYPRINUS-CARPIO L) .2. THE PRODUCTION OF HOMOZYGOUS GYNOGENETIC CLONES AND F1 HYBRIDS
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DOI：
10.1016/0044-8486(91)90015-y
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发表时间：
1991-01-15
期刊：
影响因子：
4.5
通讯作者：
HUISMAN, EA
中科院分区：
文献类型：
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作者：
KOMEN, J;BONGERS, ABJ;HUISMAN, EA
鲤鱼 (Cyprinus carpio L.) 的纯合雌核发育鱼苗是由热激卵产生的，在第一次有丝分裂中期用紫外线照射的精子（1:3 稀释，10 ml，2200 J m-2 min-1）激活。 当受精后 28-30 分钟将卵在 40 摄氏度下电击 2 分钟时，可以获得 5-15% 存活的雌核发育鱼苗的稳定产量。 三个隐性突变体色素基因的孟德尔分离模式证明了雌核发育鱼苗的纯合性。 纯合自交系是通过纯合雌核雌性的杂合雌核繁殖（第二极体保留）产生的，而F1杂种是通过这些雌性与纯合雌核雄性兄弟姐妹杂交产生的。 这些菌株的克隆性质通过接受相互交换的同种异体皮肤移植物得到明确证明。
Homozygous gynogenetic fry of common carp (Cyprinus carpio L.) were produced by heat-shocking eggs, activated with UV-irradiated sperm (1:3 diluted, 10 ml, at 2200 J m-2 min-1) during metaphase of the first mitosis. Consistent yields of 5-15% viable, gynogenetic fry were obtained when eggs were shocked at 40-degrees-C for 2 min, 28-30 min after fertilization. The homozygous nature of the gynogenetic fry was demonstrated by the mendelian segregration patterns of three recessive mutant pigment genes. Homozygous inbred strains were produced by heterozygous gynogenetic reproduction (second polar body retention) of homozygous gynogenetic females, while F1 hybrids were produced by crossing these females with homozygous gynogenetic male siblings. The clonal nature of these strains was unequivalently demonstrated by the acceptance of was unequivocally demonstrated by the acceptance of reciprocally exchanged skin allografts.