Artificial polymeric receptors on the cell surface promote the efficient cellular uptake of quantum dots
Artificial polymeric receptors on the cell surface promote the efficient cellular uptake of quantum dots
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DOI：
10.1039/c1ob05420a
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发表时间：
2011-01-01
影响因子：
3.2
通讯作者：
Ijiro, Kuniharu
中科院分区：
文献类型：
--
作者：
Niikura, Kenichi;Nambara, Katsuyuki;Ijiro, Kuniharu
在我们以前的论文中，仲胺附加的阳离子聚合物1被用作在细胞表面展示人工受体的支架（R. Kamitani等人，ChemBioChem，2009，10，230）。这种聚合物可以在细胞表面保留超过30分钟，然后缓慢内化到细胞中。在这项研究中，我们的目标是实现量子点（QDs）的有效内化到靶细胞通过人工受体的聚合物。作为受体分子，N-乙酰葡糖胺（GlcNAc）部分被引入到聚合物中，并且GlcNAc结合蛋白展示量子点被用作配体。我们发现，配体呈递量子点可以通过聚合物介导的内吞作用有效地内化到细胞中，而量子点没有内化到未经处理的细胞中。这些数据表明，我们的方法的基础上，使用聚合物的细胞表面工程提供了一种新的方法，将各种渗透性差的纳米粒子输送到细胞。
In our previous paper, secondary-amine appended cationic polymer 1 was used as a scaffold to display artificial receptors on a cell surface (R. Kamitani et al., ChemBioChem, 2009, 10, 230). This polymer can be retained on the cell surface for more than 30 min before being slowly internalized into the cells. In this study, our aim is to achieve the efficient internalization of quantum dots (QDs) into target cells via artificial receptors on the polymer. As a receptor molecule, N-acetylglucosamine (GlcNAc) moieties were introduced into the polymer, and GlcNAc binding protein-displaying QDs were used as a ligand. We found that ligand-presenting QDs could be internalized effectively into cells via polymer-mediated endocytosis, whereas QDs were not internalized into untreated cells. These data suggest that our method based on cell-surface engineering using polymers affords a new approach to the delivery of various poorly permeable nanoparticles into cells.