Upregulation of RECK gene expression by small double-stranded RNA targeting the promoter region
Upregulation of RECK gene expression by small double-stranded RNA targeting the promoter region
复制标题
DOI：
10.1038/cgt.2014.12
复制
发表时间：
2014-03
影响因子：
6.4
通讯作者：
F. Sakurai;Y. Nanjo;S. Okamoto;M. Tachibana;Hiroyuki Mizuguchi
中科院分区：
文献类型：
--
作者：
F. Sakurai;Y. Nanjo;S. Okamoto;M. Tachibana;Hiroyuki Mizuguchi
最近的研究表明，与靶基因的启动子区互补的小双链RNA（dsRNA）在转染到细胞中后增强这些基因的表达。在这里，我们表明，表达的基质金属蛋白酶（MMP）抑制剂RECK被激活的肿瘤细胞系中的转染互补的启动子的RECK基因，导致抑制MMP的表达，它抑制肿瘤细胞的侵袭。这些结果支持这样的建议，即互补于肿瘤抑制基因的启动子区的dsRNA将具有作为一种新的抗肿瘤剂的潜力。
Recent studies have demonstrated that small double-stranded RNAs (dsRNAs) complementary to the promoter region of target genes enhance the expression of those genes following transfection into cells. Here we show that expression of the matrix metalloproteinase (MMP) inhibitor RECK is activated in the cultured tumor cell lines by transfection with dsRNA complementary to the promoter of the RECK gene, leading to suppression of the expression of MMPs and it inhibited tumor cell invasion. These results support the suggestion that dsRNA complementary to the promoter region of tumor suppressor genes would have potential as a novel antitumor agent.