Polyclonal antibody directed against human RANTES ameliorates disease in the Lewis rat adjuvant-induced arthritis model
Polyclonal antibody directed against human RANTES ameliorates disease in the Lewis rat adjuvant-induced arthritis model
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DOI：
10.1172/jci2172
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发表时间：
1998-06-15
影响因子：
15.9
通讯作者：
Perez, HD
中科院分区：
文献类型：
--
作者：
Barnes, DA;Tse, J;Perez, HD
佐剂诱导的关节炎（AIA）是类风湿性关节炎的许多动物模型之一，类风湿性关节炎是一种以T淋巴细胞和巨噬细胞浸润为特征的疾病。在全血和关节中发现两种趋化因子RANTES（T淋巴细胞和单核细胞化学引诱物）和KC（中性粒细胞的化学引诱物）的水平增加。令人惊讶的是，MIP-1 α（另一种T淋巴细胞和单核细胞化学引诱物）的水平在整个疾病过程中在全血中没有变化，并且在关节中仅略微升高。RANTES表达在疾病中起重要作用，因为RANTES的多克隆抗体极大地改善了阿帕诱导的动物症状，并且发现与吲哚美辛（一种非甾体抗炎药）治疗一样有效。MIP-1 α或KC的多克隆抗体均无效，这是首次报道RANTES在AIA发展中的重要性。
Adjuvant-induced arthritis (AIA) is one of many animal models of rheumatoid arthritis, a disease characterized by a T-lymphocyte and macrophage cellular infiltrate, We have characterized the development of this disease model with respect to chemokine expression. Increased levels of two chemokines, RANTES, a T-lymphocyte and monocyte chemo-attractant, and KC a chemoattractant for neutrophils, were found in whole blood and in the joint, Surprisingly, levels of MIP-1 alpha, another T-lymphocyte and monocyte chemoattractant, were unchanged throughout the course of the disease in whole blood and only slightly elevated in the joint. RANTES expression plays an important role in the disease since a polyclonal antibody to RANTES greatly ameliorated symptoms in animals induced for APA and was found to be as efficacious as treatment with indomethacin, a non-steroidal anti inflammatory. Polyclonal antibodies to either MIP-1 alpha or KC were ineffective, This is the first report to show the importance of RANTES in the development of AIA.