Near-infrared Ag2S quantum dots-based DNA logic gate platform for miRNA diagnostics
Near-infrared Ag2S quantum dots-based DNA logic gate platform for miRNA diagnostics
复制标题
用于 miRNA 诊断的基于近红外 Ag2S 量子点的 DNA 逻辑门平台
DOI：
10.1021/acs.analchem.6b01044
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发表时间：
2016
影响因子：
7.4
通讯作者：
Fanyu Meng
中科院分区：
文献类型：
--
作者：
Peng Miao;Yuguo Tang;Bidou Wang;Fanyu Meng
miRNA表达的失调与许多疾病的发生和进展有关。这些mirna被认为是有前途的生物标志物。然而，不使用耗时的放射性标记或复杂的扩增策略来测量这些低丰度分子是具有挑战性的。在这里，我们提出了一个DNA逻辑门平台，用于近红外（NIR） Ag2S量子点（QDs）的荧光输出的miRNA诊断。精心设计的具有不同miRNA输入的支点交换介导的链位移发生在固态界面上，这控制了量子点从固态界面到溶液的释放，响应初始miRNA的多重信息。近红外Ag2S量子点优异的荧光发射特性证明了其在无扩增和灵敏的miRNA检测中的广阔前景。我们通过实现飞摩尔水平的miRNA分析和一系列逻辑电路计算的多功能性来展示该平台的潜力。
Dysregulation of miRNA expression is correlated with the development and progression of many diseases. These miRNAs are regarded as promising biomarkers. However, it is challenging to measure these low abundant molecules without employing time-consuming radioactive labeling or complex amplification strategies. Here, we present a DNA logic gate platform for miRNA diagnostics with fluorescence outputs from near-infrared (NIR) Ag2S quantum dots (QDs). Carefully designed toehold exchange-mediated strand displacements with different miRNA inputs occur on a solid-state interface, which control QDs release from solid-state interface to solution, responding to multiplex information on initial miRNAs. Excellent fluorescence emission properties of NIR Ag2S QDs certify the great prospect for amplification-free and sensitive miRNA assay. We demonstrate the potential of this platform by achieving femtomolar level miRNA analysis and the versatility of a series of logic circuits computation.