[Effect of intranasal glucosteroid on the gene expression of interleukin-5 in nasal polyps].
[Effect of intranasal glucosteroid on the gene expression of interleukin-5 in nasal polyps].
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发表时间：
2004-11
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通讯作者：
Luo Zhang;D. Han;B. Zhou;E. Fan;Zhong-yan Liu
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作者：
Luo Zhang;D. Han;B. Zhou;E. Fan;Zhong-yan Liu
目的探讨鼻内应用糖皮质激素对鼻息肉组织中白细胞介素5基因表达的影响。方法应用mRNA原位杂交技术对20例鼻息肉患者鼻息肉组织中激素治疗组和非激素治疗组的mRNA进行检测。结果鼻息肉中IL-5 mRNA阳性细胞主要为淋巴细胞或嗜酸性粒细胞。过敏组与非过敏组IL-5 mRNA阳性细胞密度分别为（12.6 ± 4.6）/0.25mm ~ 2和（14.3 ± 4.1）/0.25mm ~ 2，差异无统计学意义（t =-0.775，P > 0.05）。与对照组比较[（13.9 ± 4.2）/0.25mm2]，激素治疗组IL-5 mRNA阳性细胞密度降低[（10. 9 ± 4.2）/0.25mm2]。2 ± 3.1）/0.25mm2]，差异有统计学意义（t = 3.114，P < 0.01）。结论：激素治疗可抑制IL-5基因表达，激素可抑制嗜酸性粒细胞功能。
OBJECTIVE To investigate the effect of intranasal glucosteroid treatment on the expression of interleukin (IL)-5 gene in nasal polyps. METHODS Nasal polyps from topical steroid treated patients (n = 20) and untreated patients (n = 20) were investigated with the technique of mRNA in situ hybridization. RESULTS The majority of IL-5 mRNA positive cells in nasal polyps were lymphocytes or eosinophils. No statistical significance was found in the densities of IL-5 mRNA positive cells between allergic patients [(12.6 +/- 4.6)/0.25mm2] and nonallergic patients [(14.3 +/- 4.1)/0.25mm2] (t = -0.775, P > 0.05). Compared with the control group [(13.9 +/- 4.2)/0.25mm2], the density of IL-5 mRNA positive cells was decreased in the steroid-treated group [(10. 2 +/- 3.1)/0.25mm2], and the difference reached statistical significance (t = 3.114, P < 0.01). CONCLUSIONS These findings indicate that topical steroid treatment may suppress the IL-5 gene expression, and steroids may inhibit eosinophil functions.