Molecular and biophysical properties of voltage-gated Na+ channels in murine vas deferens.
Molecular and biophysical properties of voltage-gated Na+ channels in murine vas deferens.
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小鼠输精管电压门控钠通道的分子和生物物理特性。
DOI：
10.1529/biophysj.107.117192
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发表时间：
2008
影响因子：
3.4
通讯作者：
N. Teramoto
中科院分区：
文献类型：
--
作者：
Hai‐Lei Zhu;M. Aishima;H. Morinaga;R. Wassall;Atsushi Shibata;K. Iwasa;M. Nomura;M. Nagao;K. Sueishi;T. C. Cunnane;N. Teramoto
应用膜片钳技术和分子生物学方法研究了小鼠输精管肌细胞河豚毒素（TTX）敏感性电压门控Na（+）电流（I（Na））的生物学和分子生物学特性。在全细胞配置中，在Cd（2+）存在下诱发快速、瞬时内向电流，并被TTX（K（d）= 11.2 nM）、米贝拉地尔（K（d）= 3.3 μ M）和用单价阳离子（TEA（+）、Tris（+）和NMDG（+））外部置换Na（+）消除。电压门控Na（+）通道激动剂藜芦碱可增强快速瞬时内向电流，提示快速瞬时内向电流是一种TTX敏感的I（Na）。I（Na）的半数最大（V（half））失活和活化值分别为-46.3 mV和-26.0 mV。RT-PCR分析显示Scn 1a、2a和8a转录本的表达。在平滑肌层中检测到Na（V）1.6的Scn 8a转录本和α亚基蛋白。使用缺乏Na（+）通道基因表达的Na（V）1.6-null小鼠（Na（V）1.6（-/-）），在分散的输精管平滑肌细胞中未检测到Scn 8a，I（Na），而在其野生型（Na（V）1.6（+/+））同窝仔中记录到TTX敏感的I（Na）。这项研究表明，负责TTX敏感的I（Na）在小鼠输精管肌细胞的电压门控Na（+）通道的分子身份主要是Na（V）1.6。
The biological and molecular properties of tetrodotoxin (TTX)-sensitive voltage-gated Na(+) currents (I(Na)) in murine vas deferens myocytes were investigated using patch-clamp techniques and molecular biological analyses. In whole-cell configuration, a fast, transient inward current was evoked in the presence of Cd(2+), and was abolished by TTX (K(d) = 11.2 nM), mibefradil (K(d) = 3.3 microM), and external replacement of Na(+) with monovalent cations (TEA(+), Tris(+), and NMDG(+)). The fast transient inward current was enhanced by veratridine, an activator of voltage-gated Na(+) channels, suggesting that the fast transient inward current was a TTX-sensitive I(Na). The values for half-maximal (V(half)) inactivation and activation of I(Na) were -46.3 mV and -26.0 mV, respectively. RT-PCR analysis revealed the expression of Scn1a, 2a, and 8a transcripts. The Scn8a transcript and the alpha-subunit protein of Na(V)1.6 were detected in smooth muscle layers. Using Na(V)1.6-null mice (Na(V)1.6(-/-)) lacking the expression of the Na(+) channel gene, Scn8a, I(Na) were not detected in dispersed smooth muscle cells from the vas deferens, while TTX-sensitive I(Na) were recorded in their wild-type (Na(V)1.6(+/+)) littermates. This study demonstrates that the molecular identity of the voltage-gated Na(+) channels responsible for the TTX-sensitive I(Na) in murine vas deferens myocytes is primarily Na(V)1.6.
影响因子：
3.4
作者：
W. Zhou;A. Goldin
通讯作者：
W. Zhou;A. Goldin
米贝拉地尔对钠电流和钙电流的影响。
DOI：
10.1152/ajpgi.00022.2005
发表时间：
2005
期刊：
American journal of physiology. Gastrointestinal and liver physiology
影响因子：
--
作者：
Strege,PeterR;Bernard,CherylE;Ou,Yijun;Gibbons,SimonJ;Farrugia,Gianrico
通讯作者：
Farrugia,Gianrico