Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses
Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses
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DOI：
10.1006/viro.1997.8606
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发表时间：
1997-06-23
期刊：
影响因子：
3.7
通讯作者：
Emerman, M
中科院分区：
文献类型：
--
作者：
Vodicka, MA;Goh, WC;Emerman, M
CCR5和CXCR4是人类免疫缺陷病毒(HIV)进入的两个主要辅助受体。我们已经对几种基于β-半乳糖苷酶的HIV指示细胞株进行了修饰，以表达CCR5和/或CXCR4。使用这些新的试剂，我们已经能够检测到使用这些细胞系中的一个或两个进行测试的所有初级分离株。然而，病毒的绝对感染力在不同的原始毒株之间有很大的差异。此外，当存在辅助受体时，所有HIV毒株都能够在指示细胞中引起合胞体，无论它们以前是被描述为“合胞体诱导”还是“非合胞体诱导”。(C)1997年学术出版社。
CCR5 and CXCR4 are the two major coreceptors that have been identified for human immunodeficiency virus (HIV) entry. We have modified several beta-galactosidase-based HIV indicator cell lines to express CCR5 and/or CXCR4. Using these new reagents, we have been able to detect all primary isolates tested using one or both of these cell lines. However, there is large variation in the absolute viral infectivity among primary strains. Furthermore, all HIV strains are capable of causing syncytia in the indicator cells when the coreceptor is present regardless of whether they had previously been characterized as ''syncytia-inducing'' or ''non-syncytium-inducing.'' (C) 1997 Academic Press.