Immobilization of amine-modified oligonucleotides on aldehyde-terminated alkanethiol monolayers on gold
Immobilization of amine-modified oligonucleotides on aldehyde-terminated alkanethiol monolayers on gold
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DOI：
10.1021/la048166r
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发表时间：
2005-01-04
期刊：
影响因子：
3.9
通讯作者：
Smith, LM
中科院分区：
文献类型：
--
作者：
Peelen, D;Smith, LM
描述了用于在金表面上制造DNA阵列的化学。用末端醛基修饰的烷硫醇用于制备自组装单分子膜（SAM）。单层的醛基可以与胺修饰的寡核苷酸或其他带有胺的生物分子反应以形成席夫碱，然后可以通过用氰基硼氢化钠处理将其还原为稳定的仲胺。的表面修饰和反应的特点是偏振调制傅里叶变换红外反射吸收光谱（PM-FTIRRAS），和可及性，结合特异性，和DNA修饰的表面的稳定性在杂交实验中证明。
Chemistry is described for the fabrication of DNA arrays on gold surfaces. Alkanethiols modified with terminal aldehyde groups are used to prepare a self-assembled monolayer (SAM). The aldehyde groups of the monolayer may be reacted with amine-modified oligonucleotides or other amine-bearing biomolecules to form a Schiff base, which may then be reduced to a stable secondary amine by treatment with sodium cyanoborohydride. The surface modifications and reactions are characterized by polarization modulation Fourier transform infrared reflection absorption spectroscopy (PM-FTIRRAS), and the accessibility, binding specificity, and stability of the DNA-modified surfaces are demonstrated in hybridization experiments.