RASSF1A methylation as a biomarker for detection of colorectal cancer and hepatocellular carcinoma.
RASSF1A methylation as a biomarker for detection of colorectal cancer and hepatocellular carcinoma.
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DOI：
10.4251/wjgo.v14.i8.1574
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发表时间：
2022-08-15
影响因子：
3
通讯作者：
Li, Zhi
中科院分区：
文献类型：
--
作者：
Li, Jian;Li, Huan;Run, Zeng-Ci;Wang, Zhen-Lei;Jiang, Tao;An, Yang;Li, Zhi
研究已经验证了甲基化游离DNA作为各种肿瘤生物标志物的潜力，血浆中的甲基化DNA可能是癌症的潜在生物标志物。探讨血浆RASSF 1A甲基化水平对结直肠癌和肝细胞癌的诊断价值。 共纳入92例CRC患者、67例结直肠息肉（CRP）患者、63例HCC患者和66例肝硬化（LC）患者。对血浆DNA进行DNA提取、双链DNA浓度测定、亚硫酸氢盐转化、纯化、单链DNA浓度测定和数字聚合酶链反应（PCR）检测。计算甲基化率。以曲线下面积（AUC）评价其诊断价值。CRC和CRP组以及HCC和LC组的年龄和性别也匹配。CRC组血浆RASSF 1A基因甲基化率为2.87 ± 1.80，CRP组为1.50 ± 0.64。血浆RASSF 1A基因甲基化水平在CRC组和CRP组间差异有统计学意义。用于区分CRC和CRP组的RASSF 1A甲基化的AUC为0.82（0.76-0.88）。T1、T2、T3和T4 CRC和CRP的AUC分别为0.83（0.72-0.95）、0.87（0.78-0.95）、0.86（0.77-0.95）和0.75（0.64-0.85）。肝细胞癌组血浆RASSF 1A基因甲基化率为4.45 ± 2.93，肝细胞癌组为2.46 ± 2.07。HCC组和LC组血浆中RASSF 1A的DNA甲基化显示出显著差异。用于区分HCC和LC组的RASSF 1A甲基化的AUC为0.70（0.60-0.79）。T1、T2、T3和T4 HCC和LC的AUC分别为0.80（0.61，1.00）、0.74（0.59-0.88）、0.60（0.42-0.79）和0.68（0.53-0.82）。数字PCR检测血浆RASSF 1A甲基化可能是CRC和HCC的潜在生物标志物。
Studies have validated the potential of methylated cell-free DNA as a biomarker in various tumors, and methylated DNA in plasma may be a potential biomarker for cancer. To evaluate the diagnostic value of RASSF1A methylation in plasma for colorectal cancer (CRC) and hepatocellular carcinoma (HCC). A total of 92 CRC patients, 67 colorectal polyp (CRP) patients, 63 HCC patients, and 66 liver cirrhosis (LC) patients were enrolled. The plasma DNA was subjected to DNA extraction, double-strand DNA concentration determination, bisulfite conversion, purification, single-strand DNA concentration determination, and digital polymerase chain reaction (PCR) detection. The methylation rate was calculated. The diagnostic value was evaluated by the area under the curve (AUC). The age and sex in the CRC and CRP groups and the HCC and LC groups were also matched. The DNA methylation rate of RASSF1A in plasma in the CRC group was 2.87 ± 1.80, and that in the CRP group was 1.50 ± 0.64. DNA methylation of RASSF1A in plasma showed a significant difference between the CRC and CRP groups. The AUC of RASSF1A methylation for discriminating the CRC and CRP groups was 0.82 (0.76-0.88). The AUCs of T1, T2, T3 and T4 CRC and CRP were 0.83 (0.72-0.95), 0.87 (0.78-0.95), 0.86 (0.77-0.95), and 0.75 (0.64-0.85), respectively. The DNA methylation rate of RASSF1A in plasma in the HCC group was 4.45 ± 2.93, and that in the LC group was 2.46 ± 2.07. DNA methylation of RASSF1A in plasma for the HCC and LC groups showed a significant difference. The AUC of RASSF1A methylation for discriminating the HCC and LC groups was 0.70 (0.60-0.79). The AUCs of T1, T2, T3 and T4 HCC and LC were 0.80 (0.61, 1.00), 0.74 (0.59-0.88), 0.60 (0.42-0.79), and 0.68 (0.53-0.82), respectively. RASSF1A methylation in plasma detected by digital PCR may be a potential biomarker for CRC and HCC.
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影响因子：
4.4
作者：
Song, Fei;Mahmood, Saleh;Ghosh, Srimoyee;Liang, Ping;Smiraglia, Domminic J.;Nagase, Hiroki;Held, William A.
通讯作者：
Held, William A.
影响因子：
3
作者：
Wang Y;Chen PM;Liu RB
通讯作者：
Liu RB
影响因子：
14.9
作者：
Li W;Li Q;Kang S;Same M;Zhou Y;Sun C;Liu CC;Matsuoka L;Sher L;Wong WH;Alber F;Zhou XJ
通讯作者：
Zhou XJ
影响因子：
--
作者：
Hesson LB;Cooper WN;Latif F
通讯作者：
Latif F
影响因子：
8.8
作者：
Danese E;Minicozzi AM;Benati M;Montagnana M;Paviati E;Salvagno GL;Gusella M;Pasini F;Guidi GC;Lippi G
通讯作者：
Lippi G