Effects of nitric oxide on skin burn wound healing.
Effects of nitric oxide on skin burn wound healing.
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一氧化氮对皮肤烧伤伤口愈合的影响。
DOI：
10.1097/bcr.0b013e3181848119
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Murad,Ferid
中科院分区：
文献类型：
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作者：
Zhu,Haifeng;Wei,Xiaofei;Bian,Ka;Murad,Ferid
越来越多的证据表明，一氧化氮（NO）在皮肤修复和重建中的重要作用。本研究采用一种新研制的NO凝胶，观察了NO对小鼠Ⅱ度烧伤创面的影响。采用常规HE染色和免疫组化方法，观察了NO对小鼠创伤愈合过程中创面愈合、毛囊再生、胶原沉积、血管生成和炎性细胞浸润的影响。NO处理显著加速了50%的再上皮化，这导致了比对照组显著更快的伤口闭合。NO显著促进卵泡干细胞募集，这是上皮再形成的关键因素。此外，小鼠毛囊再生也被NO处理增强。正如我们在大鼠模型中所报道的，NO治疗显著增加了表达前胶原的成纤维细胞的数量，其在烧伤后第10天达到峰值。我们还证明了在伤口愈合过程中，与对照组相比，NO治疗伤口中的血管生成增加。最后，我们发现NO凝胶促进了伤口床浸润和炎症细胞的保留，这些细胞是愈合过程中生长因子和细胞因子的主要来源。这些观察结果表明，从局部制剂释放的NO具有通过调节皮肤中的许多细胞过程来增强烧伤伤口愈合的潜力。
Increasing evidence showed the important role of nitric oxide (NO) in skin repair and reconstruction. In this report, we investigated the effects of NO on 2nd degree burn wound of mice with a newly developed topical NO-gel. Using regular hematoxylin&eosin staining and immunohistochemistry, we determined the effects of NO on wound closure, hair follicle regeneration, collagen deposition, angiogenesis, and inflammatory cell infiltration in the wound of mice during wound healing. NO treatment significantly accelerated re-epithelialization by 50%, which has resulted in a markedly faster wound closure than that in control group. NO significantly promoted follicle stem cell recruitment, a key player in re-epithelialization. In addition, hair follicle regeneration also was enhanced by NO treatment in mice. As we have reported with rat model, NO treatment significantly increased the number of procollagen-expressing fibroblasts, which peaked by day 10 after burn wound. We also demonstrated an increase of angiogenesis in NO treated wounds compared with that in the control group during wound healing. Finally, we found that the NO gel promoted wound bed infiltration and retention of inflammatory cells that are a major source of growth factors and cytokines during the healing processes. These observations suggest that NO released from a topical preparation has the potential to enhance burn wound healing by regulation of many cellular processes in the skin.