Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology.
Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology.
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DOI：
10.1042/bst20160052
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发表时间：
2016-06-15
影响因子：
3.9
通讯作者：
Elani Y
中科院分区：
文献类型：
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作者：
Elani Y
近几十年来，使用简单的构建模块自下而上构建人工细胞的探索受到了广泛关注，这是合成生物学的重大挑战之一。被脂质膜包裹的细胞模拟物是一类特别强大的人工细胞，这是由于它们的生物相容性和在其中重建生物机器的能力。该领域的关键障碍之一集中在以下方面：如何以可控的方式和高通量地产生基于膜的人工细胞？特别是，它们如何被构造成具有精确定义的参数，包括大小，生物分子组成和空间组织？微流体生成策略已被证明有助于解决这些问题。本文将概述一些基于膜的人工细胞及其使用微流体构建的主要原理，并将详细介绍最近已经实现的一些里程碑。
The quest to construct artificial cells from the bottom-up using simple building blocks has received much attention over recent decades and is one of the grand challenges in synthetic biology. Cell mimics that are encapsulated by lipid membranes are a particularly powerful class of artificial cells due to their biocompatibility and the ability to reconstitute biological machinery within them. One of the key obstacles in the field centres on the following: how can membrane-based artificial cells be generated in a controlled way and in high-throughput? In particular, how can they be constructed to have precisely defined parameters including size, biomolecular composition and spatial organization? Microfluidic generation strategies have proved instrumental in addressing these questions. This article will outline some of the major principles underpinning membrane-based artificial cells and their construction using microfluidics, and will detail some recent landmarks that have been achieved.