Genetic analysis of lysosomal trafficking in Caenorhabditis elegans
Genetic analysis of lysosomal trafficking in Caenorhabditis elegans
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DOI：
10.1091/mbc.e05-01-0060
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发表时间：
2005-07-01
影响因子：
3.3
通讯作者：
Priess, JR
中科院分区：
文献类型：
--
作者：
Hermann, GJ;Schroeder, LK;Priess, JR
秀丽隐杆线虫胚胎的肠细胞中含有明显的双折射肠颗粒，我们发现这些颗粒是溶酶体相关的细胞器。肠道颗粒被溶酶体标记物标记，在缺乏AP-3亚基、VPS-16和VPS-41的胚胎中，它们的形成被破坏。我们定义了一类肠颗粒缺失（glo）突变体，它们在肠颗粒生物发生方面有缺陷。我们发现，格洛-1基因编码预测的拉布GTdR定位于溶酶体相关的肠道颗粒在肠道和格洛-4编码一个可能的格洛-1鸟嘌呤核苷酸交换因子。这些和其他glo基因与参与特定溶酶体相关细胞器（如哺乳动物中的黑素体和果蝇中的色素颗粒）生物发生的基因同源。因此，glo突变体提供了一个简单的模型系统，用于分析动物细胞中溶酶体相关的细胞器生物发生。
The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule loss (glo) mutants that are defective in gut granule biogenesis. We show that the glo-1 gene encodes a predicted Rab GTPase that localizes to lysosome-related gut granules in the intestine and that glo-4 encodes a possible GLO-1 guanine nucleotide exchange factor. These and other glo genes are homologous to genes implicated in the biogenesis of specialized lysosome-related organelles such as melanosomes in mammals and pigment granules in Drosophila. The glo mutants thus provide a simple model system for the analysis of lysosome-related organelle biogenesis in animal cells.