Hyalocytes synthesize and secrete inhibitors of retinal pigment epithelial cell proliferation in vitro
Hyalocytes synthesize and secrete inhibitors of retinal pigment epithelial cell proliferation in vitro
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DOI：
10.1001/archopht.1996.01100130723015
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发表时间：
1996-06-01
影响因子：
--
通讯作者：
Campochiaro, PA
中科院分区：
文献类型：
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作者：
Lazarus, HS;Schoenfeld, CL;Campochiaro, PA
背景：视网膜色素上皮（RPE）细胞在病理状态下进入玻璃体，如视网膜脱离，可能增殖并有助于视网膜前膜的形成。目的：探讨内源性玻璃体细胞透明质细胞是否在RPE细胞增殖过程中起调节作用。方法：用培养的牛透明细胞培养的培养基孵育后，用氚化胸腺嘧啶掺入密度捕获的人RPE细胞，测量细胞增殖。在透明细胞条件培养基中进行了抑制活性的初步表征，包括转化生长因子β (2) （tgf - β）中和抗体的阻断实验和MV-1-Lu水貂肺上皮细胞的增殖实验。Northern blot检测透明细胞tgf - β信使RNA的表达。结果：在存在或不存在血清或蛋白酶抑制剂的情况下，透明质细胞条件培养基抑制RPE细胞和mv -1水貂肺上皮细胞中氚化胸腺嘧啶的掺入。部分抑制活性被一种针对tgf - β的抗体中和。透明细胞RNA的Northern印迹显示存在tgf - β信使RNA(2)。这些数据表明，tgf - β负责透明细胞分泌的抑制活性的一部分。额外的抑制活性可归因于一种或多种不同于tgf - β的低分子量分子。结论：透明细胞条件培养基通过tgf - β和至少一种其他分子抑制RPE细胞的体外增殖。这些因子在体内由透明细胞产生，可以阻止在病理条件下可能受到干扰的视网膜前膜形成。
Background: Retinal pigment epithelial (RPE) cells that enter the vitreous in pathologic conditions, such as retinal detachment, may proliferate and contribute to the formation of epiretinal membranes.Objective: To study whether hyalocytes, endogenous vitreous cells, play a role in modulating the proliferation of RPE cells.Methods: Cell proliferation was measured by tritiated thymidine incorporation in density-arrested human RPE cells after incubation with media that had been conditioned by cultured bovine hyalocytes. Preliminary characterization of inhibitory activity in hyalocyte-conditioned medium was performed, including blocking experiments with a neutralizing antibody to transforming growth factor-beta(2) (TGF-beta) and proliferation assays that used MV-1-Lu mink lung epithelial cells. Northern blots were done to assess hyalocyte expression of TGF-beta messenger RNA.Results: Hyalocyte-conditioned medium inhibited tritiated thymidine incorporation in RPE cells and MV-1-Lu mink lung epithelial cells in the presence or absence of serum or protease inhibitors. A portion of the inhibitory activity was neutralized by an antibody directed against TGF-beta. Northern blots of hyalocyte RNA demonstrated the presence of messenger RNA for TGF-beta(2). These data suggest that TGF-beta is responsible for a portion of the inhibitory activity secreted by hyalocytes. Additional inhibitory activity is attributable to one or more low-molecular-weight molecules distinct from TGF-beta.Conclusion: Hyalocyte-conditioned medium inhibits RPE cell proliferation in vitro through TGF-beta and at least one other molecule. Production of these factors by hyalocytes in vivo could provide a deterrent for epiretinal membrane formation that may be perturbed under pathologic conditions.