COMPARISON OF MUSCLE-FIBER TYPING BY QUANTITATIVE ENZYME ASSAYS AND BY MYOSIN ATPASE STAINING
COMPARISON OF MUSCLE-FIBER TYPING BY QUANTITATIVE ENZYME ASSAYS AND BY MYOSIN ATPASE STAINING
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DOI：
10.1177/32.6.6202737
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发表时间：
1984-01-01
影响因子：
3.2
通讯作者：
LOWRY, OH
中科院分区：
文献类型：
--
作者：
HINTZ, CS;COYLE, EF;LOWRY, OH
使用组织化学 ATP 酶染色对人和大鼠肌肉横截面中的纤维进行分类，并将结果与从连续冻干切片中解剖的相同纤维片段的定量酶测定结果进行比较。在每种情况下测量了先前用于评估代谢类型的两种酶：乳酸脱氢酶和腺苷激酶（人纤维）或苹果酸脱氢酶（大鼠纤维）。对于人类纤维，ATP 酶染色和代谢酶测定在区分 I 型和 II 型纤维方面基本上完全一致。对于 IIA 型和 IIB 型光纤，这一一致性不太一致。在 1 块人体肌肉中鉴定出许多 ATP 酶 IIC 型纤维，根据代谢酶测定结果发现这些纤维介于 ATP 酶 I 型和 IIA 型之间。来自跖肌的大鼠纤维 ATP 酶 I、IIA 和 IIB 型在二维乳酸脱氢酶-苹果酸脱氢网格上分离得相当好。在大鼠比目鱼肌中，I 型 ATP 酶和 IIA 型纤维转移至较低的乳酸脱氢酶水平，IIC 纤维插入它们之间。
Fibers in cross sections of human and rat muscle were typed by using histochemical ATPase stains, and the results were compared with those of quantitative enzyme assays of fragments of the same fibers dissected from serial freeze-dried sections. Two enzymes previously used to assess the metabolic type were measured in each case: lactate dehydrogenase and either adenylokinase (human fibers) or malate dehydrogenase (rat fibers). With human fibers there was essentially complete agreement between ATPase staining and the metabolic enzyme assays in distinguishing types I and II fibers. The agreement was less consistent with regard to type IIA and IIB fibers. A number of ATPase type IIC fibers were identified in 1 human muscle, and were found to fall between ATPase types I and IIA on the basis of metabolic enzyme assay results. Rat-fiber ATPase types I, IIA and IIB from the plantaris muscle were rather well segregated on a 2-dimensional lactate dehydrogenase-malate dehydrogense grid. In the rat soleus muscle, ATPase types I and IIA fibers were shifted to lower lactate dehydrogenase levels, with IIC fibers interposed between them.