Dimorphic Expression of dmrt1 and cyp19a1 (Ovarian Aromatase) during Early Gonadal Development in Pejerrey, Odontesthes bonariensis
Dimorphic Expression of dmrt1 and cyp19a1 (Ovarian Aromatase) during Early Gonadal Development in Pejerrey, Odontesthes bonariensis
复制标题
DOI：
10.1159/000195681
复制
发表时间：
2008-01-01
影响因子：
2.3
通讯作者：
Somoza, G. M.
中科院分区：
文献类型：
--
作者：
Fernandino, J. I.;Hattori, R. S.;Somoza, G. M.
佩杰里鱼 (Odontesthes bonariensis) 是一种具有强烈温度依赖性性别决定 (TSD) 的硬骨鱼。多项研究表明，dmrt1 和性腺芳香酶 (cyp19a1) 与硬骨鱼的性别分化过程有关，但对于 TSD 期间这两个基因的表达平衡和内分泌调节知之甚少。本研究旨在阐明在雌性、雄性和混合性别产生温度（分别为 FPT、MPT 和 MixPT）下饲养的佩杰瑞犬性腺性别分化过程中这两个基因的表达模式。与 FPT 相比，MPT 时性腺性别分化过程中 dmrt1 的表达显着更高。相反，在 FPT 分化期间，cyp19a1 表达明显增加，但在 MPT 时则没有。 MixPT 中两个基因的表达均显示出二态性特征，其个体值类似于 MPT 或 FPT 中的值。无论温度如何，外源 17β-雌二醇的施用分别下调和上调 dmrt1 和 cyp19a1 的表达，并在 MPT 时挽救了雌性表型。然而，用芳香酶抑制剂法屈唑治疗会导致男性化，但不会改变基因表达模式。这些结果有力地证明了这两个基因参与了佩杰瑞的性腺分化过程。讨论了雌二醇的参与。版权所有 (c) 2009 S. Karger AG，巴塞尔
The pejerrey (Odontesthes bonariensis) is a teleost fish with strong temperature-dependent sex determination (TSD). Several studies have shown that dmrt1 and gonadal aromatase (cyp19a1) are implicated in the sex differentiation process in teleosts but little is known on the expression balance and endocrine regulation of these two genes during TSD. This study was designed to clarify the expression patterns of both genes during gonadal sex differentiation of pejerrey reared at female-, male- and mixed-sex-producing temperatures (FPT, MPT, and MixPT, respectively). The expression of dmrt1 was found to be significantly higher during gonadal sex differentiation at MPT compared to FPT. Conversely, cyp19a1 expression clearly increased during differentiation at FPT but not at MPT. The expression of both genes at MixPT showed a dimorphic profile with individual values resembling either those at the MPT or FPT. Administration of exogenous 17 beta-estradiol down- and up-regulated the expression of dmrt1 and cyp19a1, respectively, regardless of temperature, and rescued the female phenotype at the MPT. However, treatment with the aromatase inhibitor Fadrozole caused masculinization without changing the pattern of gene expression. These results are strong evidence of the involvement of both genes in the gonadal differentiation process of pejerrey. The involvement of estradiol is discussed. Copyright (c) 2009 S. Karger AG, Basel