MAPRE2 regulates the first meiotic progression in mouse oocytes.
MAPRE2 regulates the first meiotic progression in mouse oocytes.
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DOI：
10.1016/j.yexcr.2022.113135
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发表时间：
2022-04
影响因子：
3.7
通讯作者：
Yuan-Yuan Li-Yuan;Wen-Long Lei;Chang-Fa Zhang;Si-Min Sun;B. Zhao;Ke Xu;Yi Hou;Yingchun Ouyang;Zhenbo Wang;Lei Guo;Qing-Yuan Sun;Zhiming Han
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文献类型：
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作者：
Yuan-Yuan Li-Yuan;Wen-Long Lei;Chang-Fa Zhang;Si-Min Sun;B. Zhao;Ke Xu;Yi Hou;Yingchun Ouyang;Zhenbo Wang;Lei Guo;Qing-Yuan Sun;Zhiming Han
微管加末端跟踪蛋白（+TIPs）与生长的微管加末端相关，并控制微管动力学以及在细胞分裂、细胞迁移和形态发生期间与不同细胞结构的相互作用。微管相关RP/EB家族成员2（MAPRE 2/EB 2）是+TIPs网络的高度保守的核心组分，但该分子是否为哺乳动物减数分裂进程所需尚不清楚。在这项研究中，我们研究了MAPRE 2在卵母细胞成熟过程中的表达和功能。结果表明，MAPRE 2在胚泡期（GV）至中期II（MII）的卵母细胞中持续表达，在GV期分布于卵母细胞胞质中，在中期I（MI）和MII期分布于纺锤体的沿着。小干扰RNA介导的Mapre 2敲低严重损害微管稳定性、着丝粒-微管附着和染色体排列，随后引起纺锤体组装检查点（SAC）激活和细胞周期蛋白B1非降解，导致染色体分离失败和第一极体排出。本研究首次证明MAPRE 2在小鼠卵母细胞减数分裂过程中发挥重要作用。
Microtubule plus-end tracking proteins (+TIPs) associate with growing microtubule plus ends and control microtubule dynamics and interactions with different cellular structures during cell division, cell migration and morphogenesis. Microtubule-associated RP/EB family member 2 (MAPRE2/EB2) is a highly conserved core component of +TIPs networks, but whether this molecule is required for mammalian meiotic progression is unknown. In this study, we investigated the expression and function of MAPRE2 during oocyte maturation. Our results showed that MAPRE2 was consistently expressed from germinal vesicle (GV) to metaphase II (MII) stages and that MAPRE2 was distributed in the cytoplasm of oocytes at GV stage and along the spindle at metaphase I (MI) and MII stages. Small interfering RNA-mediated knockdown ofMapre2severely impaired microtubule stability, kinetochore-microtubule attachment, and chromosome alignment and subsequently caused spindle assembly checkpoint (SAC) activation and cyclin B1 nondegradation, leading to failure of chromosome segregation and first polar body extrusion. This study demonstrates for the first time that MAPRE2 plays an important role during mouse oocyte meiosis.