Regulation of Nicotine Tolerance by Quorum Sensing and High Efficiency of Quorum Quenching Under Nicotine Stress in Pseudomonas aeruginosa PAO1.
Regulation of Nicotine Tolerance by Quorum Sensing and High Efficiency of Quorum Quenching Under Nicotine Stress in Pseudomonas aeruginosa PAO1.
复制标题
铜绿假单胞菌 PAO1 尼古丁胁迫下群体感应和高效群体淬灭对尼古丁耐受的调节
DOI：
10.3389/fcimb.2018.00088
复制
发表时间：
2018
影响因子：
5.7
通讯作者：
Wang M
中科院分区：
文献类型：
--
作者：
Tang H;Zhang Y;Ma Y;Tang M;Shen D;Wang M
群体感应（Quorum sensing，QS）调节细菌种群的行为，促进它们在压力下的适应和生存。由于QS对绝大多数细菌的毒力负责，因此群体淬灭（QQ），即QS的中断，已成为一种有吸引力的治疗策略。然而，QS在胁迫耐受中的作用以及QQ在胁迫下在细菌中的效率很少被探索。在这项研究中，我们证明了QS调节过氧化氢酶（CAT）的表达和生物膜的形成帮助铜绿假单胞菌PAO 1抵抗尼古丁胁迫。野生型（WT）和ΔrhlR菌株的CAT活性和生物膜形成显著高于ΔlasR菌株。用3 OC 12-HSL补充ΔlasI菌株显示出与WT菌株相似的CAT活性和生物膜形成。尼古丁耐受的关键是LasIR回路而不是RhlIR回路。酰化酶I显着降低尼古丁胁迫下的毒力因子，即弹性蛋白酶，绿脓菌素，绿脓菌荧光素的生产相比，在没有尼古丁胁迫下观察到的水平。因此，QQ在压力下更有效率。据我们所知，这是第一项报告QS有助于铜绿假单胞菌尼古丁耐受性的研究。这项工作有助于更好地应用QQ治疗细菌感染，特别是在压力下。
Quorum sensing (QS) regulates the behavior of bacterial populations and promotes their adaptation and survival under stress. As QS is responsible for the virulence of vast majority of bacteria, quorum quenching (QQ), the interruption of QS, has become an attractive therapeutic strategy. However, the role of QS in stress tolerance and the efficiency of QQ under stress in bacteria are seldom explored. In this study, we demonstrated that QS-regulated catalase (CAT) expression and biofilm formation help Pseudomonas aeruginosa PAO1 resist nicotine stress. CAT activity and biofilm formation in wild type (WT) and ΔrhlR strains are significantly higher than those in the ΔlasR strain. Supplementation of ΔlasI strain with 3OC12-HSL showed similar CAT activity and biofilm formation as those of the WT strain. LasIR circuit rather than RhlIR circuit is vital to nicotine tolerance. Acylase I significantly decreased the production of virulence factors, namely elastase, pyocyanin, and pyoverdine under nicotine stress compared to the levels observed in the absence of nicotine stress. Thus, QQ is more efficient under stress. To our knowledge, this is the first study to report that QS contributes to nicotine tolerance in P. aeruginosa. This work facilitates a better application of QQ for the treatment of bacterial infections, especially under stress.