Biochemical composition and dynamics of the axonal cytoskeleton in the corticospinal system of the adult hamster.
Biochemical composition and dynamics of the axonal cytoskeleton in the corticospinal system of the adult hamster.
复制标题
成年仓鼠皮质脊髓系统轴突细胞骨架的生化组成和动力学。
DOI：
10.1007/bf01001353
复制
发表时间：
1988
影响因子：
3.6
通讯作者：
Oblinger,MM
中科院分区：
文献类型：
--
作者：
Oblinger,MM
皮质脊髓系统是一个重要的中枢神经系统（CNS）通路，与肌萎缩性侧索硬化症等衰弱性疾病和脊髓创伤性损伤有关。这项研究的特点正常皮质脊髓轴突的一些基本的生化和动力学特性，建立一个重要的参考研究，旨在阐明这些轴突的病理条件下导致的细胞修饰。慢轴突运输传递轴突细胞骨架以及细胞基质成分，如许多代谢酶和调节蛋白，已被检查。在这些研究中，将[35 S]蛋氨酸注射到成年雄性金黄仓鼠的感觉运动皮质中，并使用一维和二维凝胶电泳/荧光照相术评估注射后1-42天皮质脊髓轴突中存在的标记转运蛋白。在成年皮质脊髓轴突中，观察到慢组分B（SC B）蛋白（包括网格蛋白、肌动蛋白、烯醇化酶、肌酸磷酸激酶和许多其他蛋白）的复杂组以约2 mm/天的速率移动。慢组分a（SCa）蛋白（微管蛋白、神经丝蛋白和肌动蛋白）以大约0.4 mm/天的显著较慢的速率运输。皮质脊髓轴突中慢转运的生化和动力学性质与先前在另一个CNS途径中描述的那些非常相似，视网膜神经节细胞的轴突，并且与在大的外周感觉或运动轴突中记录的那些实质上不同。这些研究结果表明，轴突运输的一些基本特性，决定了许多轴突的结构和功能特性可能是不同的中枢神经系统相比，周围神经系统。
The corticospinal system is an important central nervous system (CNS) pathway that is implicated in debilitating diseases such as amyotrophic lateral sclerosis and in traumatic injuries to the spinal cord. This study characterizes some of the fundamental biochemical and kinetic properties of normal corticospinal axons, establishing an important reference for studies that aim to elucidate the cellular modifications that result during pathological conditions of these axons. Slow axonal transport which conveys the axonal cytoskeleton as well as cytomatrix constituents, such as many of the metabolic enzymes and regulatory proteins, has been examined. For these studies, [35S]methionine was injected into the sensorimotor cortex of adult male Golden hamsters, and labeled, transported proteins present in corticospinal axons at 1–42 days after injection were assessed using one- and two-dimensional gel electrophoresis/fluorography. The complex group of slow component b (SCb) proteins (including clathrin, actin, enolase, creatine phosphokinase, and many others) was observed to move at a rate of approximately 2 mm/day in adult corticospinal axons. The slow component a (SCa) proteins (tubulins, neurofilament proteins, and actin) were transported at a substantially slower rate of approximately 0.4 mm/day. The biochemical and kinetic properties of slow transport in corticospinal axons were very similar to those previously described in another CNS pathway, axons of retinal ganglion cells, and substantially different from those documented in large, peripheral sensory or motor axons. These findings suggest that some of the basic properties of axonal transport which determine many of the structural and functional properties of axons may be different in the CNS compared to the peripheral nervous system.