Detection and genetic characterization of peste des petits ruminants virus in free-living bharals (Pseudois nayaur) in Tibet, China
Detection and genetic characterization of peste des petits ruminants virus in free-living bharals (Pseudois nayaur) in Tibet, China
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DOI：
10.1016/j.rvsc.2010.05.031
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发表时间：
2011-04-01
影响因子：
2.4
通讯作者：
Qi, Lin
中科院分区：
文献类型：
--
作者：
Bao, Jingyue;Wang, Zhiliang;Qi, Lin
小反刍兽疫（PPR）是绵羊和山羊的一种重要病毒性疾病。小反刍兽疫的野生动物宿主可能在该疾病的流行病学中发挥重要作用，但其特征尚不明确。这项研究的目的是研究中国西藏自由生活的印度虎（Pseudois nayaur）中 PPR 病毒（PPRV）的感染情况。 2007年，根据临床症状和组织样本中PPRV RNA的检测，在西藏日土县的两个原住民中确认了PPRV感染。 2008年，通过竞争性ELISA、逆转录聚合酶链反应以及PPRV融合蛋白（F）和核蛋白（N）基因片段的序列分析，在西藏格杰县的一处原野发现了PPRV感染。在受感染的印度羊中发现的 PPRV 变种与最近在西藏绵羊和山羊中发现的其他流行的 PPRV 变种密切相关。这是自由生活的原住民中首次报告 PPRV 感染。 (C) 2010 年，爱思唯尔有限公司出版。
Peste des petits ruminants (PPR) is an important viral disease of sheep and goats. The wildlife hosts of PPR, which may play an important role in the epidemiology of this disease, are not well characterized. The research was undertaken to study the infection of PPR virus (PPRV) in free-living bharals (Pseudois nayaur) in Tibet, China. In 2007, PPRV infection was confirmed in two bharals in Rutog County of Tibet based on clinical signs and detection of PPRV RNA in tissue samples. In 2008, PPRV infection was found in one bharal in Ge'gyai County of Tibet by competitive ELISA, reverse transcription-polymerase chain reaction, and sequence analysis of PPRV fusion protein (F) and nucleoprotein (N) gene segments. The PPRV variant identified in infected bharal was closely related to other circulating PPRV variants recently identified in sheep and goats from Tibet. This is the first report of PPRV infection in free-living bharals. (C) 2010 Published by Elsevier Ltd.