The Process of Apoptosis in a Holocrine Gland as Shown by the Avian Uropygial Gland
The Process of Apoptosis in a Holocrine Gland as Shown by the Avian Uropygial Gland
复制标题
DOI：
10.1002/ar.22645
复制
发表时间：
2013-03-01
影响因子：
2
通讯作者：
Alan, Emel
中科院分区：
文献类型：
--
作者：
Liman, Narin;Alan, Emel
本研究旨在阐明鸡尾臀腺，一个专门的全分泌分泌腺内的细胞凋亡和定位的凋亡相关的Bax和生存素蛋白和增殖细胞核抗原（PCNA）的存在。在日龄雏鸡中，Survivin和Bax的免疫反应观察到的细胞质中的萌发和分泌层的腔上皮和小管。在此期间，TUNEL反应，细胞凋亡的指示，只是零星阳性的小管。从孵化后第7天到第150天，在腺体的萌发层细胞的细胞质和分泌层的一些细胞的细胞核中检测到Survivin。萌发层细胞Bax呈弱的均匀胞质染色，而腔上皮和小管的分泌层和中间层的细胞呈颗粒状胞质染色。第7天后，在腔上皮和中央小管的分泌和变性层中观察到TUNEL阳性细胞。第12天后，在外周小管中也观察到一些TUNEL阳性细胞。在所有的postthatch年龄，细胞质和细胞核的萌发层的腔上皮和小管与PCNA反应，而只有少数细胞核的分泌层是免疫阳性。这些结果支持了这样的理论，即特定的PCNA/Bax/Survivin表达模式可以反映特定的细胞分化状态在尾腺和腺的全分泌主要是通过细胞凋亡的方式实现。Anat Rec，296：504-520，2013. (C)2013 Wiley Periodicals，Inc.
This study was designed to elucidate the presence of apoptosis and the localization of apoptosis-related Bax and survivin proteins and proliferating cell nuclear antigen (PCNA) within the chicken uropygial gland, a specialized holocrine secretory gland. In day-old chicks, survivin and Bax immunoreactivities were observed in the cell cytoplasm of the germinative and secretory layers of the luminal epithelium and tubules. During this period, the TUNEL reaction, an indication of apoptosis, was only sporadically positive in the tubules. From the 7th day to the 150th day of posthatching, survivin was detected in the cytoplasm of cells in the germinative layer and in the nuclei of some cells in the secretory layers of the gland. The germinative layer cells showed weak homogeneous cytoplasmic staining for Bax, whereas the cells of the secretory and intermediate layers of luminal epithelium and tubules exhibited granular cytoplasmic staining. After day 7, TUNEL-positive cells were observed in the secretory and degenerative layers of the luminal epithelium and central tubules. After day 12, some TUNEL-positive cells were also seen in the peripheral tubules. At all posthatch ages, the cytoplasm and nucleus of the germinative layers of luminal epithelium and tubules reacted with PCNA, whereas only a small number of cell nuclei in the secretory layers were immunopositive. These results support the theory that specific PCNA/Bax/survivin expression patterns could reflect particular cell differentiation states in the uropygial gland and that holocrine secretion in the gland is realized mainly by way of apoptosis. Anat Rec, 296: 504-520, 2013. (C) 2013 Wiley Periodicals, Inc.