Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode
Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode
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DOI：
10.2116/analsci.20.1143
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发表时间：
2004-08
影响因子：
1.6
通讯作者：
Y. Kase;H. Muguruma
中科院分区：
文献类型：
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作者：
Y. Kase;H. Muguruma
我们提出了一种基于等离子体聚合薄膜的电子转移介导安培型酶生物传感器的溅射金电极上的二甲基氨基甲基二茂铁（DMAMF）。DMAMF等离子体聚合膜在干燥条件下直接沉积在电极表面上。所得薄膜不仅具有氧化还原位点，而且非常薄（约20 nm），很好地粘附在基底（电极）上，具有平坦的表面和高度交联的网络结构，并且本质上是亲水性的。葡萄糖氧化酶被致密地固定在金电极上的DMAMF等离子体聚合膜的表面上。从电化学测量来看，该生物传感器可以覆盖较宽的葡萄糖浓度范围（1。3-81 mM）。葡萄糖生物传感器的电流响应降低小于5%，在有氧溶液中相比，在厌氧溶液中。这表明DMAMF等离子体聚合膜在酶反应中心和电极之间起着电子传递介质的作用。
We propose an electron transfer-mediated amperometric enzyme biosensor based on plasma-polymerized thin film of dimethylaminomethylferrocene (DMAMF) on a sputtered gold electrode. The DMAMF plasma-polymerized film is deposited directly onto the surface of the electrode under dry conditions. The resulting thin film not only has redox sites but also is extremely thin (~20 nm), adheres well onto the substrate (electrode), has a flat surface and a highly-crosslinked network structure, and is hydrophilic in nature. Glucose oxidase is densely immobilized onto the surface of DMAMF plasma-polymerized film on the gold electrode. From the electrochemical measurement, the biosensor can cover the wide range of glucose concentration (1. 3–81 mM) at +350 mV of applied potential. The current response of the glucose biosensor was decreased by less than 5% in an aerobic solution as compared to that in an anaerobic solution. These show that the DMAMF plasma-polymerized films play a role as the electron transfer mediators between the reaction center of enzyme and the electrode.