Exploring the functional soil-microbe interface and exoenzymes through soil metaexoproteomics.
Exploring the functional soil-microbe interface and exoenzymes through soil metaexoproteomics.
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DOI：
10.1038/ismej.2014.130
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发表时间：
2014-10
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影响因子：
--
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中科院分区：
文献类型：
--
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与通常回收的细胞内蛋白质相比，在细胞和土壤界面处的功能重要的蛋白质具有潜在的低丰度。开发了一种新的方法，并用于提取的metaexoproteome，细胞外发现的蛋白质的子集，在富含含氮的柠檬酸聚合物甲壳素的土壤的背景下。回收的大多数蛋白质是细菌来源的，并且定位于外膜或细胞外环境。鉴定了多种转运蛋白，特别是与氨基酸和磷酸盐摄取相关的转运蛋白。后外蛋白质组提取物保留了几丁质分解活性，并且我们成功地检测了与糖苷水解酶家族18（GH18）chi基因数据和后分类学分析相关的诺卡氏菌样几丁质酶。诺卡氏菌样几丁质酶似乎是唯一负责几丁质在土壤中的活性。这是第一个检测和测序土壤酶库中具有已证实活性的细菌胞外酶的研究。
Functionally important proteins at the interface of cell and soil are of potentially low abundance when compared with commonly recovered intracellular proteins. A novel approach was developed and used to extract the metaexoproteome, the subset of proteins found outside the cell, in the context of a soil enriched with the nitrogen-containing recalcitrant polymer chitin. The majority of proteins recovered was of bacterial origin and localized to the outer membrane or extracellular milieu. A wide variety of transporter proteins were identified, particularly those associated with amino-acid and phosphate uptake. The metaexoproteome extract retained chitinolytic activity and we were successful in detecting Nocardiopsis-like chitinases that correlated with the glycoside hydrolase family 18 (GH18) chi gene data and metataxonomic analysis. Nocardiopsis-like chitinases appeared to be solely responsible for chitinolytic activity in soil. This is the first study to detect and sequence bacterial exoenzymes with proven activity in the soil enzyme pool.
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