Acute PAR2 activation reduces GABAergic inhibition in the spinal dorsal horn
Acute PAR2 activation reduces GABAergic inhibition in the spinal dorsal horn
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急性 PAR2 激活减少脊髓背角的 GABA 抑制
DOI：
10.1016/j.brainres.2011.09.058
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发表时间：
2011-11-24
期刊：
影响因子：
2.9
通讯作者：
Lu, Zhijie
中科院分区：
文献类型：
--
作者：
Huang, Zhangxiang;Tao, Kunming;Lu, Zhijie
我们研究了抑制脊髓背角GABA能神经传递的机制，以阐明蛋白酶激活受体-2(PAR2)的作用。最初，我们证实鞘内注射PAR2激动剂SL-NH2会产生机械性痛敏。然后，我们在脊髓切片的胶状质神经元上进行了膜片钳实验，发现PAR2激动剂SL-NH2短暂孵育(2min)后，神经元的自发突触后抑制电流(SIPSCs)的频率和幅度都显著降低。在SL-NH2处理过程中，GABA介导的电流显著减少，而对甘氨酸介导的电流没有影响。这些结果表明，激活PAR2增强了痛反应，可能是通过抑制背角GABA能神经传递。(C)2011爱思唯尔B.V.保留所有权利。
We investigated the mechanism underlying inhibition of spinal dorsal horn GABAergic neurotransmission to elucidate the role of protease-activated receptor-2 (PAR2). Initially, we confirmed that PAR2 agonist SL-NH2 applied intrathecally produced mechanical hyperalgesia. Then we performed patch-clamp experiments in substantia gelatinosa neurons of spinal cord slice, and found that spontaneous inhibitory post-synaptic currents (sIPSCs) were significantly decreased in both frequency and amplitude when neurons were incubated with PAR2 agonist SL-NH2 for a brief time period (2 min). The GABA-mediated currents were significantly reduced, and there was no impact on glycine-mediated currents during this SL-NH2 treatment. These results suggest that PAR2 activation enhanced the pain response, potentially via inhibition of dorsal horn GABAergic neurotransmission. (C) 2011 Elsevier B.V. All rights reserved.