A GAL4-Driver Line Resource for Drosophila Neurobiology
A GAL4-Driver Line Resource for Drosophila Neurobiology
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DOI：
10.1016/j.celrep.2012.09.011
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发表时间：
2012-10-01
期刊：
影响因子：
8.8
通讯作者：
Zugates, Christopher T.
中科院分区：
文献类型：
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作者：
Jenett, Arnim;Rubin, Gerald M.;Zugates, Christopher T.
我们建立了一个包含7000个黑腹果蝇转基因品系的集合。每个品系中GAL4的表达由基因组DNA的一个不同的、确定的片段控制，该片段作为转录增强子。我们使用解剖后的神经系统的共聚焦显微镜来确定每个片段在成年果蝇大脑和腹神经索中驱动的表达模式。我们展示了6650个品系的图像数据。通过手动和机器辅助注释，我们描述了最有用的品系中的表达模式。我们举例说明了这些数据在识别新型神经元细胞类型、揭示大脑不对称性以及描述神经元形状刻板性的性质和程度方面的用途。GAL4品系允许外源基因在成年神经系统的不同的、小的子集中表达。这组每个都驱动一种有记录的表达模式的DNA片段，将有助于产生用于操纵神经元功能的其他构建体。
We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.